A hyperspectral reflectance and fluorescence micro-endoscopic system for diagnostics and therapy

这项研究的重点是对乳汁、胰腺、胆管等难以接近的导管中的癌症或其他病理损害进行光谱成像检测。乳腺、胆管、金色膀胱、胰腺的恶性肿瘤通常发生在导管上皮内，因此可通过导管发现。这些恶性肿瘤大多是侵袭性的，为及时诊断和成功的手术干预留下了很短的时间(几个月)。该项目的主要目标是开发和测试一种通用的研究型显微内窥镜系统。工作目标包括开发一种基于声光滤光器的可调光谱波段内窥镜光源，用于光谱反射成像和激光二极管阵列，用于激发-发射成像数据收集，以促进组织形态参数、细胞新陈代谢和局部血液含量的成像。利用所提出的光学技术进行的生物组织检查将与病理组织学等传统诊断技术相结合进行验证和改进。通过这项研究，将在学术-临床-工业环境下培养一名高素质的生物光子学人才。预计该项目将有助于发展战略方向，更好地了解导管病理发展的基本机制，微型高分辨率成像技术，新一代显微内窥镜技术，包括同时引导和监测的内窥镜光疗，开发真实的组织模型以减少对实验动物的使用，并可能导致微型内窥镜视频设施和超分辨率(亚波长域)内窥镜模式的发展。

The focus of the study is a spectral imaging detection of cancer or other pathological lesions in hardly approachable ducts such as breast milk, pancreatic, Biliary ducts. The malignancies in breasts, Biliary ducts, gold bladder, pancreas are frequently developed within the duct epithelium, and hence detectable via ducts. Most of these malignancies are aggressive leaving a short time (months) for in-time diagnostics and successful consequent surgical interventions. The primary aim of the project is to develop and test a versatile research micro-endoscopic system. The work objectives include development of a tunable spectral band endoscopic light source based on acousto-optical filter for spectral reflectance imaging and laser diode array for excitation-emission imaging data collection to facilitate imaging of tissue morphological parameters, cell metabolism and local blood content. The biotissue examination by mean of the optical technology proposed will be combined with traditional diagnostic technique such as pathohistology for validation and improvement. As a result of the study, a high-qualified personal will be trained in the field of Biophotonics in academic-clinical-industrial environment. It is anticipated that the project will contribute into the development of a strategical direction bringing better understanding of the basic mechanisms of pathological development in ducts, microscale high resolution imaging technique, new generation micro-endoscopy technology including endoscopic light therapy with simultaneous guidance and monitoring, development of realistic tissue phantoms to reduce usage of lab animals and may lead to the development of the micro-endoscopic video facility and super resolution (subwavelength domain) endoscopic modalities.