Assessment of peptide and amino acid profiles following simulated dynamic gastrointestinal model digestion of collagen hydrolysate

Genacol Canada Corporation Inc.生产一种牛来源的水解胶原蛋白补充剂(Genacol®原始配方)，该补充剂已在两个单独的临床试验中显示出缓解关节疼痛的效果。经过消化，水解胶原蛋白产生生物可利用的氨基酸和多肽，这可以导致补充剂的健康促进特性。然而，目前还没有关于牛源Genacol胶原蛋白水解物消化产生的多肽和氨基酸图谱的信息，因为这些化合物的性质和图谱将根据用于生产胶原蛋白水解物的加工类型和胶原蛋白的食物来源而有很大不同。在本研究中，将使用动态模拟肠道消化模型来研究Genacol胶原蛋白产品在消化酶介导的水解过程中产生的多肽和氨基酸的分布。这种方法是确定Genacol胶原蛋白补充剂促进健康特性的生物活性成分的第一步，并将有助于开发更集中和更有效的水解胶原蛋白产品。

Genacol Canada Corporation Inc. manufactures a bovine-sourced hydrolyzed collagen supplement (Genacol®Original Formula), which has shown efficacy in two separate clinical trials for reducing joint pain. Followingdigestion, hydrolyzed collagen yields bioavailable amino acids and peptides that can lead to the healthpromoting properties of the supplement. There is presently no information, however, regarding the peptide andamino acid profiles generated from the digestion of the bovine-sourced Genacol collagen hydrolysate as thenature and profiles of these compounds will differ greatly depending upon of the type of processing used toproduce the collagen hydrolysate and the food source of the collagen. In the present study, the profiles of thepeptides and amino acids generated from digestive enzyme-mediated hydrolysis of the Genacol collagenproduct will be investigated using the dynamic simulated gut digestion model. This approach is the first step toascertain the bioactive components contributing towards the health promoting properties of the Genacolcollagen supplement and will aid in the development of more concentrated and potent hydrolyzed collagenproducts.