Handheld Multiphoton Imaging Device for Detecting Oral Cancer

根据加拿大癌症协会的数据，口腔癌的5年生存率只有63%。加拿大每年有4600例新病例，全世界每年有30万例新病例，平均一半的患者在确诊后5年内死亡。口腔癌术后复发率高达20%。因此，迫切需要**能够提高口腔癌的早期发现和治疗的新技术。本项目将开发一种基于多光子成像的新型即时性肿瘤成像设备，为口腔黏膜病变提供高分辨率的活体实时成像。多光子成像可以为癌症检测提供重要生物标志物的可视化：肿瘤细胞和细胞核的形态变化，以及底层胶原纤维的密度和顺序，这些都有助于肿瘤细胞的进展和侵袭。它在动物模型的临床前癌症研究中被证明是非常成功的。将多光子成像**转化为临床应用才刚刚开始。挑战包括处理飞秒增益和有限的信噪比。我们将解决这些挑战，设计一种便携式多光子系统，配备手持探针，用于口腔黏膜的体内成像。**将对患者进行体内成像，以评估该设备的引导活检和**肿瘤边缘检测。这项新技术可以通过对肿瘤进行无创实时评估，并指导何时何地进行活检以进行早期癌症检测，从而改善癌症管理。

According to the Canadian Cancer Society, the 5-year survival rate for oral cancer is only**63%. Each year 4,600 new cases in Canada and 300,000 cases worldwide are identified and**on average, half of the patients die within 5 years of diagnosis. The recurrence rate of oral**cancer after surgery is high at 20%. Therefore, there is a critical need for new technology that**can improve the early detection and treatment of oral cancer. In this project, a new point-ofcare**cancer imaging device based on multiphoton imaging will be developed to provide highresolution**imaging of oral mucosa lesions in vivo and in real-time. Multiphoton imaging can**provide visualization of important biomarkers for cancer detection: the morphological changes**of tumor cells and nuclei, and the density and ordering of the underlying collagen fibers which**facilitates the progression and invasion of tumor cells. It has been demonstrated to be very**successful in pre-clinical cancer studies on animal models. Translating multiphoton imaging**into clinical applications is just beginning. Challenges include handling femtosecond pluses**and limited signal to noise ratio. We will address those challenges and design a portable**multiphoton system with a handheld probe for performing in vivo imaging of the oral mucosa.**In vivo imaging on patients will be carried out to evaluate the device for guided biopsy and**tumor margin detection. The new technology can improve cancer management by real-time**assessment of tumor noninvasively and guiding decisions on when and where to take**biopsies for early cancer detection.