Multimode determination of gene transcription and metabolic labeling

核受体是配体活化蛋白家族的一部分，其存在于细胞核中并控制参与代谢功能的不同方面的特定基因的表达。为了实现这种控制，核受体直接参与与靶DNA的接触，以激活或抑制细胞中的特定基因组。因此，研究控制代谢途径各个步骤的关键基因如何通过核受体激活进行调节，将大大提高我们对基因转录和动力学的理解。在实验室中，我们特别研究了两种雌激素受体（ERa，ERb）和PPAR家族成员，基于它们各自调节对激素的反应和控制能量代谢的潜力。拟议的设备是必不可少的，以调查的功能方面和特定的核受体的基因转录，复杂的组装，线粒体活性和细胞表型的背景下修改。该设备还将允许对共同申请人进行的其他研究项目进行特定的代谢测量。因此，几个实验室将支持几种细胞生物学方法，以解决每个研究项目中关键效应物和条件的作用和影响。使用拟议的设备后，还将加快组建和培训高素质人员的工作。我们的长期目标是提高我们对即将到来的信号如何影响细胞过程的理解，以便在控制基因网络和代谢结果方面提供新的见解。

Nuclear receptors are part of a family of ligand activated proteins that reside in the nucleus of cells and control the expression of specific genes involved in diverse aspects of metabolic functions. To achieve such control, nuclear receptors are directly involved in making contact with target DNA in order to activate or repress specific sets of genes in cells. As such, investigation of how key genes controlling various steps of metabolic pathways are regulated through nuclear receptor activations will greatly improve our understanding of gene transcription and dynamics.***In the laboratory, we have been studying in particular the two estrogen receptors (ERa, ERb) and the PPAR family members based on their respective potential to modulate response to hormones and to control energy metabolism. The proposed equipment is essential to investigate the functional aspects and specific modifications of nuclear receptors in the context of gene transcription, complex assembly, mitochondrial activity and cell phenotypes. The equipment will also allow specific metabolic measurements in respect to other research projects undertaken by the co-applicants. Therefore, several cell biology approaches will be supported in several laboratories to address the role and impact of key effectors and conditions in every research project. The formation and training of highly qualified personnel will also be favorized with the use of the proposed equipment.***Our long term objective is to improve our understanding of how upcoming signals impact cellular processes, in order to provide novel insights in the control of gene network and metabolic outcomes.