Liquid Chromatography High Resolution Tandem Mass Spectrometry for Proteomics, Metabolomics, Epigenetics, and Synthetic Oligonucleotides.

我们申请资金用于购买高压液相色谱高分辨率串联质谱系统（HPLC-HR-MS/MS）。******该仪器系统对安大略省理工大学（Ontario Tech）水生组学实验室Denina Simmons博士的研究项目的各个方面都至关重要，该项目由加拿大研究主席计划（Tier II）和NSERC（发现资助、发现加速器补充和发射补充）资助。请求的系统还将从根本上扩展Jean-Paul Desaulniers博士的既定研究计划，该计划由NSERC的四个项目（发现基金（DG）， 2个合作研究发展基金（CRD）和1个I2I基金）资助。****** HPLC-HR-MS/MS系统将用于大规模和高通量测序，测量和表征存在于复杂生物血浆和组织样品中的大分子和小分子（即寡核苷酸，蛋白质，代谢物和药物），合成反应后相对纯净的溶液或环境基质（如水和沉积物）。没有其他可用的仪器适合于如此多样化和苛刻的分析，附近具有类似能力的设施没有足够的时间来支持博士所需的小时数。Simmons博士和Desaulniers博士所要求的灵活性，以执行新的生物分析方法开发。因此，延迟购买该设备将对Simmons博士的研究计划的启动和Desaulniers博士已建立的研究的扩展造成重大障碍。******博士。西蒙斯的研究项目侧重于环境影响监测的非致死方法；她探索了家庭污染物、采矿和工业废物以及气候驱动的水生压力源（如有害藻华、缺氧和入侵物种）对野生鱼类健康的影响。Desaulniers博士的研究项目包括开发新型合成寡核苷酸，可用于治疗因基因过度表达而发生的疾病，并创造新的修饰，以改善递送和减少非靶标效应。******在接下来的5年中，所要求的设备将支持在Simmons博士和Desaulniers博士的联合研究小组中培训至少30名高素质人员（包括本科生）。对所有HQP的培训将包括仪器的实际使用，以及使用生物信息学和化学信息学软件对大型复杂光谱数据集进行统计数据分析和解释。Simmons博士和Desaulniers博士都努力为所有人提供培训和研究机会，并创建性别，文化和社会多样性平衡的研究团队。*****

We request funding towards the purchase of a high-pressure liquid chromatography high-resolution tandem-mass spectrometry system (HPLC-HR-MS/MS). ******This instrument system is essential to all aspects of Dr. Denina Simmons' research program in the Aquatic Omics Lab at the University of Ontario Institute of Technology (Ontario Tech), which is funded by the Canada Research Chairs program (Tier II) and NSERC (Discovery grant, Discovery Accelerator Supplement, and a Launch Supplement). The requested system will also fundamentally expand the established research program of Dr. Jean-Paul Desaulniers which is funded by four NSERC programs (Discovery Grant (DG), 2 Collaborative Research Development (CRD) Grants, and an I2I grant). ******The HPLC-HR-MS/MS system will be used to enable large-scale and high-throughput sequencing, measurement, and characterization of both large and small biomolecules (i.e. oligonucleotides, proteins, metabolites, and drugs) that are present complex biological plasma and tissue samples, in relatively pure solutions after synthesis reactions, or in environmental matrices such as water and sediment. There is no other available instrumentation that is suitable for such a diverse and demanding array of analyses, and nearby facilities with similar capabilities do not have enough time available to support the number of hours required by Drs. Simmons' research program, nor to do provide the flexiblity required by both Dr. Simmons and Dr. Desaulniers to perform new bioanalytical method development. Thus, a delay in the acquisition of this equipment will cause a substantial impediment to the start-up of Dr. Simmons' research program and the expansion of Dr. Desaulniers' established research.******Dr. Simmons' research program focuses on non-lethal methods for environmental effect monitoring; she explores the impacts of household contaminants, mining and industrial waste, as well as climate-driven aquatic stressors like harmful algal blooms, hypoxia, and invasive species on the health of wild fish populations. Dr. Desaulniers' research program involves developing novel synthetic oligonucleotides that can be used as therapeutic treatments for diseases that occur due to the over expression of genes, and creating novel modifications that improve delivery and reduce non-target effects.******Over the next 5 years, the requested equipment will support the training of at least 30 highly qualified personnel (including undergraduate students) in the combined research groups of Dr. Simmons and Dr. Desaulniers. Training for all HQP will include hands-on use of the instrument, as well as the statistical data analysis and interpretation of large and complex spectral data-sets, using bioinformatics and chemoinformatics software. Both Dr. Simmons and Dr. Desaulniers make effort provide training and research opportunities and that are inclusive to all people and create research teams that are balanced in gender, cultural, and social diversity.*****