Acquisition of a 500 MHz NMR Spectrometer

要求为购买一台共享的500兆赫核磁共振光谱仪提供部分经费。拟议的文书将大大有助于本提案中概述的九个研究项目。这些包括噬菌体T4溶菌酶的结构和动力学；噬菌体T4基因32蛋白的“锌指”结构；四烷基铵化合物与DNA相互作用的性质；酵母羧肽酶向液泡分选过程中一个决定因素的结构参与F1/F0 atp酶质子泵送的天冬氨酸残基的结构和热力学性质；底物在酶间直接转移的研究重要生物化合物的合成；辅酶B12的作用机制；以及含硒化合物对丝氨酸蛋白酶抑制的结构和机制。

Partial funding for the purchase of a shared 500 MHz NMR spectrometer is requested. The proposed instrument will greatly aid in the nine research projects outlined in this proposal. These include the structure and dynamics of bacteriophage T4 lysozyme; the structure of the "zinc finger" of bacteriophage T4 gene 32 protein; the nature of the interactions of tetraalkylammonium compounds with DNA; the structure of a determinant in the sorting of yeast carboxypeptidase to the vacuole; the structure and thermodynamic properties of an aspartic acid residue involved in proton pumping by the F1/F0 ATPase; an investigation of the direct transfer of substrates between enzymes; the synthesis of biologically important compounds; the mechanism of action of coenzyme B12; and the structure and mechanism of inhibition of serine proteases by selenoxide containing compounds.