Triple Quadrupole/SID and Pentaquadrupole Mass Spectrometers

这是一个探索新方法适用性的建议 表面诱导解离（SID）的生物学问题。 在 在这个实验中，生物化合物将被电离， 较新的解吸电离方法和电离化合物 在串联质量的第一级中选择感兴趣的 光谱仪 然后将使用与固体表面的碰撞 解离选定的生物化合物及其结构 将通过扫描记录SID子光谱来推断 第二个分析师。 这种方法的优点在于， 常规串联质谱法将通过 获取三重四极杆仪器， 离子/表面以及气相碰撞的能力。 新仪器将取代PI中的三重四极杆 实验室，提供质量范围（4000与1000道尔顿）更好 适合研究生物化合物。 在评价 SID的能力，重点将放在肽 测序 还有很多其他生物学上重要的 还将讨论包括DNA分析在内的问题， 与致癌碳氢化合物的加合物，细胞壁结构 低聚糖，以及监测产品产生的 生物反应器 除了将SID用于生物 化合物，离子/分子反应，其对 将探索特定的功能组。 的能力 将提供给普渡大学的科学家 他们有特别严重的问题。 利用将是 通过校园范围内的质谱设施协调， 和跨学科的设施，协调访问和 在校园里使用质谱仪。

This is a proposal to explore the applicability of the new method of surface-induced dissociation (SID) to biological problems. In this experiment, biological compounds will be ionized by one of the newer desorption ionization methods and the ionized compound of interest selected in the first stage of a tandem mass spectrometer. Collisions with a solid surface will then be used to dissociate the selected biological compound and its structure will be deduced by recording an SID daughter spectrum by scanning the second analyzer. The advantages of this approach over conventional tandem mass spectrometry will be evaluated by acquiring a triple quadrupole instrument which incorporates capabilities for ion/surface as well as gas phase collisions. The new instrument will replace a triple quadrupole in the PI's laboratory, providing a mass range (4000 vs. 1000 daltons) better suited to the study of biological compounds. In evaluating the capabilities of SID, emphasis will be placed on peptide sequencing. A wide variety of other biologically important problems will also be addressed including the analysis of DNA adducts with carcinogenic hydrocarbons, structures of cell wall oligosaccharides, as well as monitoring products generated in bioreactors. In addition to the use of SID for biological compounds, ion/molecule reactions which are selective for particular functional groups will be explored. The capabilities of the new instrument will be made available to Purdue scientists who have particularly significant problems. Utilization will be coordinated through the Campus-wide Mass Spectrometry Facility, and interdisciplinary facility which coordinates access to and utilization of mass spectrometers on campus.