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Recombinant Production of Hyperthermophilic Enzymes: Effect of Temperature and Pressure

Recombinant Production of Hyperthermophilic Enzymes: Effect of Temperature and Pressure
超嗜热酶的重组生产：温度和压力的影响
批准号：
9421862
负责人：
Douglas Clark
金额：
$18.13万
依托单位：
University of California-Berkeley
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1994
资助国家：
美国
项目状态：
已结题
起止时间：
1994-09-01 至 1996-08-31
来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9421862&HistoricalAwards=false
关键词：
RecombinantProductionHyperthermophilicEnzymesEffect

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中文摘要
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英文摘要
9421862 Clark This project is on research to study the production of enzymes from extremophiles by expression of these enzymes in mesophiles, using recombinant DNA technology. The objective is to make available large quantities of these enzymes for biophysical and biochemical characterization and ultimately for enzymatic applications, without the need for culturing extremophiles directly. The specific objectives of this research include: (1) to clone and identify gene sequences from the hyperthermophile Pyrococcus furiosus and related thermophiles; (2) to express genes in heterologous systems at high pressure, and characterize recombinant enzymes under elevated pressure and temperature to establish optimal specific activity and assembly; and (3) to develop bioprocessing methods for obtaining enzymes in sufficient quantity for structural determination and biotechnological applications. ***
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