Recombinant Production of Hyperthermophilic Enzymes: Effect of Temperature and Pressure

这个项目是研究利用重组DNA技术，通过在嗜酸性微生物中表达这些酶来研究从极端微生物中产生的酶。我们的目标是在不需要直接培养极端微生物的情况下，使这些酶大量用于生物物理和生化表征，并最终用于酶的应用。本研究的具体目的包括：(1)克隆并鉴定超嗜热菌furiosus及相关嗜热菌的基因序列；(2)在高压外源系统中表达基因，并在高压和高温下对重组酶进行表征，以确定最佳的比活性和组装；(3)开发生物加工方法，以获得足够数量的酶，用于结构测定和生物技术应用。＊＊＊

9421862 Clark This project is on research to study the production of enzymes from extremophiles by expression of these enzymes in mesophiles, using recombinant DNA technology. The objective is to make available large quantities of these enzymes for biophysical and biochemical characterization and ultimately for enzymatic applications, without the need for culturing extremophiles directly. The specific objectives of this research include: (1) to clone and identify gene sequences from the hyperthermophile Pyrococcus furiosus and related thermophiles; (2) to express genes in heterologous systems at high pressure, and characterize recombinant enzymes under elevated pressure and temperature to establish optimal specific activity and assembly; and (3) to develop bioprocessing methods for obtaining enzymes in sufficient quantity for structural determination and biotechnological applications. ***