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Collaborative Research: Recombinant Production of Enzymes from Hypethermophiles: Effects of High Temperature and Pressure

Collaborative Research: Recombinant Production of Enzymes from Hypethermophiles: Effects of High Temperature and Pressure
合作研究：嗜热菌酶的重组生产：高温高压的影响
批准号：
9604561
负责人：
Douglas Clark
金额：
$39.2万
依托单位：
University of California-Berkeley
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1996
资助国家：
美国
项目状态：
已结题
起止时间：
1996-09-15 至 2001-08-31
来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9604561&HistoricalAwards=false
关键词：
CollaborativeResearchRecombinantProductionEnzymes

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英文摘要
9604561 Clark This proposal is on research to examine two key issues in the development of enzymes and other proteins from thermophilic microorganisms which are to: (1) develop processing methods to obtain significant quantities of these enzymes for further investigation and potential application, and (2) examine the mechanisms of pressure-induced stabilization of proteins from extreme thermophiles and devise approaches to manipulate the thermostability of these enzymes. Site-directed mutagenesis is to be used in a systematic way, along with biocatalytic studies at elevated pressures to elucidate the effects of pressure on protein thermostability and the interaction of subunits in these multimeric enzymes. ***
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