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Integrative analysis of multiple pathways in the honeybee olfactory system

蜜蜂嗅觉系统多通路的综合分析

基本信息

批准号：
123268752
负责人：
Professor Dr. Giovanni Galizia
金额：
--
依托单位：
Lehrstuhl für Verhaltensphysiologie und Soziobiologie (Zoologie II)
依托单位国家：
德国
项目类别：
Priority Programmes
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2017-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/123268752?language=en
关键词：
Integrative analysis multiple pathways honeybee

项目摘要

A striking commonality across species is the recurring presence of parallel olfactory systems, such as the main olfactory system, the vomeronasal system, the septal organ and the Grueneberg organ in mammals. Similarly, different subsystems are present in amphibians, fish and insects, suggesting that parallel systems have a highly adaptive value. We use the honeybee Apis mellifera to study a species where two distinct olfactory subsystems of about equal size have been found within the main olfactory system: the l-APT and the m-APT pathway. In addition, the honeybee offers a large body of knowledge and a wealth of paradigms to study olfactory anatomy, physiology and behavior, including learning and memory with close to cognitive capacities. In the first funding period we found important new anatomical and physiological characteristics of this dual olfactory pathway. At the sensory input side, basiconic sensilla are sex-specific and mostly restricted to the m-APT subsystem in females, which is reduced in male drones. Ultrastructural analyses of output synapses in the mushroom body calyx revealed new aspects of synaptic plasticity in the two subsystems. Most importantly, using optophysiological recordings and real-time dual tract multiunit electrophysiology we show that both antennal-lobe output tracts respond to a similar panel of odors indicating true parallel processing. We found evidence for at least two mechanisms of synaptic plasticity in the l-APT subsystem following associative odor learning. Consequently, our current and future physiological experiments focus on these parallel coding properties and their plasticity by using an expanded panel of odor stimuli. Future analyses largely focus on spatial and temporal response properties (latency and coincidence code) using different odor concentrations and mixtures. In addition, we will continue to probe the neurochemical characteristics and intrinsic properties of neurons belonging to the two pathways and test their behavioral relevance. We expect to improve our understanding of parallel olfactory coding mechanisms and of the significance of parallel pathways in sensory systems in general.

一个惊人的共同点是重复存在的平行嗅觉系统，如主要的嗅觉系统，犁鼻系统，隔器官和Grueneberg器官在哺乳动物。同样，不同的子系统存在于两栖动物，鱼类和昆虫，这表明并行系统具有高度的适应价值。我们使用蜜蜂蜜蜂研究一个物种，其中两个不同的嗅觉子系统的大小相等的主要嗅觉系统内已被发现：L-APT和M-APT途径。此外，蜜蜂提供了大量的知识和丰富的范式来研究嗅觉解剖学，生理学和行为，包括学习和记忆与认知能力。在第一个资助期间，我们发现了这种双重嗅觉通路的重要新的解剖学和生理学特征。在感觉输入侧，锥形感器是性别特异性的，主要限于在女性的m-APT子系统，这是减少在男性雄蜂。超微结构分析的输出突触在蘑菇体花萼揭示了新的方面的突触可塑性的两个子系统。最重要的是，使用光生理记录和实时双道多单位电生理，我们表明，两个触角叶输出道响应类似的面板的气味，表明真正的并行处理。我们发现的证据，至少有两种机制的突触可塑性的l-APT子系统联想气味学习。因此，我们目前和未来的生理实验集中在这些并行编码的特性和它们的可塑性，通过使用扩大面板的气味刺激。未来的分析主要集中在空间和时间响应特性（潜伏期和重合代码）使用不同的气味浓度和混合物。此外，我们将继续探索属于这两个途径的神经元的神经化学特征和内在特性，并测试它们的行为相关性。我们希望提高我们的理解，并行嗅觉编码机制和平行通路的意义，在感觉系统一般。