A 600 MHz NMR Spectrometer for Biomolecule Analysis

摘要本项目为加州理工学院化学与化学工程学部仪器设备采购一台600 MHz核磁共振光谱仪。这种高场仪器的主要目的是测定生物合成、半合成和合成来源的复杂生物分子的结构。该司若干小组的研究活动将大大受益于新的光谱仪，在许多情况下，新的仪器将使全新的项目成为可能。600兆赫光谱仪具有在5至30 kD范围内确定配合物结构所需的分辨率和灵敏度。需要该仪器的研究包括金属- dna复合物的研究；蛋白质和肽- dna复合物；DNA插入物的结构；红霉素的超热稳定性研究；新型蛋白质的结构与动力学；人工蛋白质;离子通道蛋白；以及野生型和突变型网蛋白的电子转移反应。这份文书的提供将对该司的研究和培训活动产生重大影响。

9877039Abstract This project involves the acquisition of a 600 MHz NMR spectrometer for the Instrumentation facilities of the Division of Chemistry and Chemical Engineering of California Institute of Technology. The major purpose of this high field instrument will be for the determination of the structures of complex biological molecules of biosynthetic, semisynthetic and synthetic origin. The research activities of several groups in the Division will benefit greatly from the new spectrometer and in many cases, entirely new projects will be made possible by the new instrument. The 600 MHz spectrometer has the resolution and sensitivity needed to determine structures of complexes in the 5 to 30 kD range. Research for which the instrument is required includes the study of metal-DNA complexes; protein- and peptide-DNA complexes; the structure of DNA intercalators; studies of the hyperthermostability of rubredoxin from P. furiosus; structure and dynamics of novel proteins; artificial proteins; ion channel proteins; and electron transfer reactions in wild-type and mutant netalloproteins. The availability of this instrument will have a major impact on both the research and training activities of the Division.