A 600 MHz NMR Spectrometer for Biomolecule Analysis

这个项目涉及为加州理工学院化学和化学工程部的仪器设备购买一台600 MHz的核磁共振波谱仪。这台高场仪器的主要用途是测定生物合成、半合成和合成来源的复杂生物分子结构。该司几个小组的研究活动将大大受益于新的光谱仪，在许多情况下，新仪器将使全新的项目成为可能。600 MHz光谱仪具有确定5至30 kD范围内的络合物结构所需的分辨率和灵敏度。需要该仪器的研究包括金属-DNA络合物的研究；蛋白质-DNA络合物的研究；DNA嵌入器的结构；来自P.Furiosus的Rubredoxin的超热稳定性的研究；新蛋白质的结构和动力学；人造蛋白质；离子通道蛋白质；以及野生型和突变型Netallation蛋白中的电子转移反应。这一工具的提供将对该司的研究和培训活动产生重大影响。

9877039Abstract This project involves the acquisition of a 600 MHz NMR spectrometer for the Instrumentation facilities of the Division of Chemistry and Chemical Engineering of California Institute of Technology. The major purpose of this high field instrument will be for the determination of the structures of complex biological molecules of biosynthetic, semisynthetic and synthetic origin. The research activities of several groups in the Division will benefit greatly from the new spectrometer and in many cases, entirely new projects will be made possible by the new instrument. The 600 MHz spectrometer has the resolution and sensitivity needed to determine structures of complexes in the 5 to 30 kD range. Research for which the instrument is required includes the study of metal-DNA complexes; protein- and peptide-DNA complexes; the structure of DNA intercalators; studies of the hyperthermostability of rubredoxin from P. furiosus; structure and dynamics of novel proteins; artificial proteins; ion channel proteins; and electron transfer reactions in wild-type and mutant netalloproteins. The availability of this instrument will have a major impact on both the research and training activities of the Division.