A MALDI Time-of-Flight Mass Spectrometer for Protein Analysis and Peptide Mapping

用于蛋白质分析和肽图分析的 MALDI 飞行时间质谱仪

• 批准号: 0099874
• 负责人: David Lubman
• 金额: $ 19.13万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-15 至 2003-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0099874&HistoricalAwards=false
• 关键词: MALDI; Time; Flight; Mass; Spectrometer

This award provides support for acquisition of a MALDI/reflectron time-of-flight massspectrometer system. This system will be used for studies of protein structure and function by a broad range of investigators including seven faculty and students in the departments Biophysics, Biology, Chemistry and Pharmacy on the main campus of the University. The system will provide the required resolution and mass accuracy to support applications in peptide mapping for identification and searching of modification sites in protein digests, and for studying various protein-protein and protein-drug interactions. A high mass detector will permit monitoring of proteins as a function of cellular processes, protein-drug studies and for verifying sequences obtained by peptide mapping. Mass spectrometry is a widely-used, critical technology in the study of protein structure and function, sometimes referred to proteomics. In addition to the group of seven major users, at 13 other investigators will benefit from the availability of the MALDI-TOF device. Many of these investigators currently rely on instruments in local industry or in private laboratories for their research. The new instrument will be located in the mass spectrometry facility in the Chemistry Department and will accessible for use by investigators from other departments. In addition to its use in research, the instrument will be used in graduate and postdoctoral training of a large number of students, including as many as 50 students and post-doctoral trainees in the major users laboratories, plus a large number of other students, including undergraduates, in these and other laboratories.

该合同为MALDI/反射器飞行时间质谱仪系统的采购提供支持。该系统将被广泛的研究人员用于蛋白质结构和功能的研究，包括大学主校区生物物理学，生物学，化学和药学系的七名教师和学生。该系统将提供所需的分辨率和质量精度，以支持肽图谱的应用，用于识别和搜索蛋白质酶中的修饰位点，以及研究各种蛋白质-蛋白质和蛋白质-药物相互作用。高质量检测器将允许监测蛋白质作为细胞过程的功能，蛋白质药物研究和验证通过肽图谱获得的序列。 质谱是蛋白质结构和功能研究中广泛使用的关键技术，有时也称为蛋白质组学。 除了7名主要用户外，还有13名其他研究人员将受益于MALDI-TOF设备的可用性。这些研究人员中有许多目前依靠当地工业或私人实验室的仪器进行研究。新仪器将位于化学系的质谱设施中，可供其他部门的研究人员使用。 除了用于研究之外，该仪器还将用于对大量学生进行研究生和博士后培训，其中包括主要用户实验室的多达50名学生和博士后受训人员，以及这些实验室和其他实验室的大量其他学生，包括本科生。