Acquisition of an LC/MS/MS System for Chemistry and Medical School Departments

北达科他大学化学系和医学院将获得一台高分辨率串联质谱仪（MS/MS， QQTOF几何），该质谱仪由主要研究仪器（MRI）项目提供，采用大气压力电离（API）源。该系统将与液相色谱（LC/MS/MS）相结合。该设备将加强若干领域的研究，包括a)鉴定植物中的含硒蛋白质和相关化合物，以及一旦它们被食用后的生物功能；B)多巴胺转运蛋白的研究；c) 4-羟基-2-壬烯醛（HNE）及其代谢产物的生物化学分析；D)鸡眼脉络膜上液的特征；e) c -糖苷、c -双糖及相关化合物的合成。液相色谱质谱检测（LC-MS）是一种非常强大的技术，用于分离和分析复杂的混合物。这将是整个北达科他州第一个具有高分辨率质谱和串联质谱能力的仪器。这些研究将对生物化学产生重大影响。

With this award from the Major Research Instrumentation (MRI) Program, the Chemistry and Medical School Departments at the University of North Dakota will acquire a high-resolution tandem mass spectrometer (MS/MS, QQTOF geometry) with an atmospheric pressure ionization (API) source. The system will be interfaced to liquid chromatography (LC/MS/MS). This equipment will enhance research in a number of areas including a) the identification of selenium-containing proteins and related compounds in plants and in biological functions once they have been consumed; b) studies on the dopamine transporter protein; c) analysis of the biochemistry of 4-hydroxy-2-nonenal (HNE) and its metabolites; d) characterization of suprachoroidal fluid in chick eyes; and e) synthesis of C-glycosides, C-disaccharides, and related compounds. Liquid chromatography with mass spectrometric detection (LC-MS) is an extremely powerful technique used for the separation and analysis of complex mixtures. This will be the first instrument with high resolution MS and tandem MS capabilities in the entire state of North Dakota. These studies will have a strong impact in biochemistry.