Acquisition of an LC/MS/MS System for Chemistry and Medical School Departments

为化学和医学院系采购 LC/MS/MS 系统

• 批准号: 0216038
• 负责人: David Pierce
• 金额: $ 25.54万
• 依托单位: University of North Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0216038&HistoricalAwards=false
• 关键词: Acquisition; LC; MS; System; Chemistry

With this award from the Major Research Instrumentation (MRI) Program, the Chemistry and Medical School Departments at the University of North Dakota will acquire a high-resolution tandem mass spectrometer (MS/MS, QQTOF geometry) with an atmospheric pressure ionization (API) source. The system will be interfaced to liquid chromatography (LC/MS/MS). This equipment will enhance research in a number of areas including a) the identification of selenium-containing proteins and related compounds in plants and in biological functions once they have been consumed; b) studies on the dopamine transporter protein; c) analysis of the biochemistry of 4-hydroxy-2-nonenal (HNE) and its metabolites; d) characterization of suprachoroidal fluid in chick eyes; and e) synthesis of C-glycosides, C-disaccharides, and related compounds. Liquid chromatography with mass spectrometric detection (LC-MS) is an extremely powerful technique used for the separation and analysis of complex mixtures. This will be the first instrument with high resolution MS and tandem MS capabilities in the entire state of North Dakota. These studies will have a strong impact in biochemistry.

有了这项来自主要研究仪器（MRI）计划的奖项，北达科他州大学的化学和医学院将获得一台高分辨率串联质谱仪（MS/MS，QQTOF几何），该质谱仪具有大气压电离（API）源。 该系统将与液相色谱（LC/MS/MS）连接。这一设备将加强若干领域的研究，包括：（a）鉴定植物中的含硒蛋白质和有关化合物，以及食用后的生物功能;（B）研究多巴胺转运蛋白;（c）分析4-羟基-2-壬烯醛及其代谢物的生物化学;（d）鸡眼脉络膜上液的特性;和e）C-糖苷、C-二糖和相关化合物的合成。 液相色谱-质谱检测（LC-MS）是一种非常强大的技术，用于分离和分析复杂的混合物。 这将是整个北达科他州第一台具有高分辨率MS和串联MS能力的仪器。 这些研究将对生物化学产生重大影响。