Development of Inquiry-Based Modules in Proteomics

蛋白质组学中基于查询的模块的开发

• 批准号: 0230794
• 负责人: Eric Eberhardt
• 金额: $ 7.49万
• 依托单位: Vassar College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-15 至 2005-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0230794&HistoricalAwards=false
• 关键词: Development; Inquiry; Based; Modules; Proteomics

This project is developing laboratory course modules in the interdisciplinary area of proteomics. Proteomics is an emerging area at the interface of biology and chemistry, which affords students the opportunity to explore complex biological systems with molecular detail. The availability of organized teaching materials using a proteomics approach at the undergraduate level is limited. Vassar College and Marist College have formed a partnership to develop prototype modules in proteomics for introductory undergraduate biochemists. The new laboratory modules blend biochemistry with disciplines in molecular biology, analytical chemistry, and computer science. Students gain exposure to a wide range of biochemical techniques including electrophoresis, MALDI/TOF mass spectrometry and bioinformatics. The goal of the overall experience is to introduce beginning undergraduate biochemistry students to the emerging field of proteomics. During the first phase of the project, prototype laboratory modules are being developed for the heat and cold shock responses in E. coli. Laboratory protocols and supplementary materials are being created during this phase. During the second phase of the project, the modules are being implemented as a laboratory course at both institutions. These courses serve to establish the suitability of a proteomics laboratory experience in an undergraduate level curriculum for a diverse range of students. These pilot trials ofthese modules will allow for a comprehensive evaluation of the pedagogical effectiveness of these new proteomics laboratories and also assess the ability of these modules to prepare students to undertake careers in the life sciences.

该项目正在开发蛋白质组学跨学科领域的实验室课程模块。蛋白质组学是生物学和化学界面上的一个新兴领域，它为学生提供了用分子细节探索复杂生物系统的机会。在本科阶段使用蛋白质组学方法的有组织的教学材料的可用性是有限的。瓦萨学院和马里斯特学院已经建立了合作伙伴关系，为本科生物化学家开发蛋白质组学的原型模块。新的实验室模块将生物化学与分子生物学，分析化学和计算机科学学科融合在一起。学生将接触到广泛的生物化学技术，包括电泳，MALDI/TOF质谱和生物信息学。整体经验的目标是介绍开始本科生生物化学学生蛋白质组学的新兴领域。在该项目的第一阶段，正在为E.杆菌在这一阶段，正在制定实验室方案和补充材料。在该项目的第二阶段，这些单元正在这两个机构作为实验室课程实施。这些课程有助于建立蛋白质组学实验室经验在本科水平的课程，为不同范围的学生的适用性。这些模块的试点试验将全面评估这些新蛋白质组学实验室的教学有效性，并评估这些模块为学生从事生命科学职业做好准备的能力。