Acquisition of a LC-MS-MS for Research in Biology, Environmental Science, and Chemistry at UT Chattanooga

该奖项支持收购液相色谱（LC）系统与串联质谱仪检测（以下简称为LC-MS-MS）。这种改进的LC系统具有填充有小于2微米的高机械强度的多孔颗粒的柱。与较大颗粒填料相比，这些色谱柱具有更高的流速和更高的峰容量，从而提高了分析物分辨率和灵敏度，并减少了分析时间和溶剂使用。 该LC/MS/MS将用于检测和分离非挥发性化合物，包括分子多面体、神经递质和超低浓度（ng/L）的蛋白质。许多教师，本科生和研究生从两个部门将利用这一仪器进行研究和研究培训。这些教师和学生代表化学，遗传学，生物学，生理学，毒理学和环境科学。 此外，每年至少有6名研究生和70名本科生将使用该仪器作为其研究和教育的一部分。 以下三门实验室课程的学生将通过研究型实验室练习获得实践经验：仪器分析，毒理学和生理学。这将是大学100英里半径内唯一的LC/MS。

This award supports the acquisition of a Liquid Chromatography (LC) system with tandem Mass Spectrometer detection (herein referred to as LC-MS-MS). This improved LC system has columns packed with porous particles of high mechanical strength that are smaller than 2 microns. These columns have higher flow rates and increased peak capacity compared to larger particle packings, resulting in improvements in analyte resolution and sensitivity, and decreases in analysis time and solvent use. This LC/MS/MS will be used for detection and separation of non-volatile compounds including molecular polyhedra, neurotransmitters, and proteins at ultra-low concentrations (ng/L). Many faculty, undergraduates, and graduate students from two departments will utilize this instrument for research and research training. These faculty and students represent Chemistry, Genetics, Biology, Physiology, Toxicology, and Environmental Science. In addition, at least 6 graduate students and 70 undergraduate students will use the instrument annually as part of their research and education. Students in the following three lab courses will have hands-on experience through research-oriented laboratory exercises: Instrumental Analysis, Toxicology, and Physiology. This will be the only LC/MS within a 100 mile radius of the university.