Development of AlGaN Biosensor sensitive in physiological saline

生态旅游- 0756594。该提案针对使用场效应晶体管检测生物分子时的德拜长度问题。由于检测距离限制在10nm或更短，由于抗体长度超出德拜长度，因此尚未使用FET传感器将抗体用于靶向生物分子。研究人员采用了仅使用靶向抗体片段治疗HEMT的方法。为了实现这一目标，采用的方法包括：(1)鉴定识别MIG（分析物受体）的抗体片段（scFvs和VHH），并选择其中的scFv和VHH进行位点特异性偶联到HEMT传感表面；(2)表征scFvs和vhs修饰的mig检测hemt的特异性和敏感性，以及传感界面的化学和电子性质；(3)在尺度纳米器件上采用控制栅极或优化结构来提高灵敏度；(4)建立理论模型并进行仿真，结合实验结果进行基础认识。

CBET-0756594S. Lee, Ohio State UniversityThis proposal targets the problems of the Debye length in using FET for detection of biomolecules. Since the distance of detection is limited to 10 nm or less, antibodies have not been used to target biomolecules using FET sensors since antibody length is beyond the Debye length. The investigators have taken the approach of using just the targeting antibody fragment for HEMT. To achieve this goal, the approaches taken include: (1) to identify antibody fragments (scFvs and VHH) that recognize MIG (analyte receptors) and engineer selected scFv and VHH thereof for site specific conjugation to HEMT sensing surfaces; (2) to characterize specificity and sensitivity of MIG-detecting HEMTs decorated with scFvs and VHHs and the chemical and electronic properties of sensing interfaces; (3) to use a control gate or an optimized structure on scaled nano-device for sensitivity enhancement and (4) to establish theoretical models and perform simulations, coupled with experimental results, for fundamental understanding.