Development of AlGaN Biosensor sensitive in physiological saline

开发对生理盐水敏感的AlGaN生物传感器

• 批准号: 0756594
• 负责人: Stephen Lee
• 金额: $ 35万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0756594&HistoricalAwards=false
• 关键词: Development; AlGaN; Biosensor; sensitive; physiological

CBET-0756594S. Lee, Ohio State UniversityThis proposal targets the problems of the Debye length in using FET for detection of biomolecules. Since the distance of detection is limited to 10 nm or less, antibodies have not been used to target biomolecules using FET sensors since antibody length is beyond the Debye length. The investigators have taken the approach of using just the targeting antibody fragment for HEMT. To achieve this goal, the approaches taken include: (1) to identify antibody fragments (scFvs and VHH) that recognize MIG (analyte receptors) and engineer selected scFv and VHH thereof for site specific conjugation to HEMT sensing surfaces; (2) to characterize specificity and sensitivity of MIG-detecting HEMTs decorated with scFvs and VHHs and the chemical and electronic properties of sensing interfaces; (3) to use a control gate or an optimized structure on scaled nano-device for sensitivity enhancement and (4) to establish theoretical models and perform simulations, coupled with experimental results, for fundamental understanding.

CBET-0756594S。李，俄亥俄州州立大学这一建议的目标是德拜长度的问题，在使用场效应管检测生物分子。由于检测距离被限制在10 nm或更小，抗体还没有被用于使用FET传感器靶向生物分子，因为抗体长度超过德拜长度。研究人员已经采取了仅使用针对HEMT的靶向抗体片段的方法。为了达到这一目的，所采取的方法包括：（1）鉴定抗体片段（scFv和VHH），识别（分析物受体）并工程化选择的scFv和VHH，用于位点特异性偶联到HEMT传感表面;（2）表征修饰有scFv和VHH的检测MIG的HEMT的特异性和灵敏度以及传感界面的化学和电子性质;（3）使用控制栅极或在缩放的纳米器件上的优化结构用于灵敏度增强，以及（4）建立理论模型并结合实验结果进行模拟，用于基本理解。