Development of AlGaN Biosensor sensitive in physiological saline

CBET-0756594S。俄亥俄州立大学李：这项提案旨在解决使用场效应管检测生物分子时的德拜长度问题。由于检测距离被限制在10 nm或更小，由于抗体的长度超过了德拜长度，所以抗体没有被用于使用FET传感器来靶向生物分子。研究人员采取了只使用针对HEMT的靶向抗体片段的方法。为实现这一目标，所采取的方法包括：(1)鉴定识别MIG(分析物受体)的抗体片段(scFv和VHH)，并将其选定的scFv和VHH用于与HEMT敏感表面的位点特异性结合；(2)表征MIG检测用scFv和VHs修饰的HEMT的特异性和灵敏度，以及传感界面的化学和电子性质；(3)使用控制门或尺度纳米器件上的优化结构来提高灵敏度；(4)建立理论模型和进行模拟，结合实验结果，以从根本上了解基础知识。

CBET-0756594S. Lee, Ohio State UniversityThis proposal targets the problems of the Debye length in using FET for detection of biomolecules. Since the distance of detection is limited to 10 nm or less, antibodies have not been used to target biomolecules using FET sensors since antibody length is beyond the Debye length. The investigators have taken the approach of using just the targeting antibody fragment for HEMT. To achieve this goal, the approaches taken include: (1) to identify antibody fragments (scFvs and VHH) that recognize MIG (analyte receptors) and engineer selected scFv and VHH thereof for site specific conjugation to HEMT sensing surfaces; (2) to characterize specificity and sensitivity of MIG-detecting HEMTs decorated with scFvs and VHHs and the chemical and electronic properties of sensing interfaces; (3) to use a control gate or an optimized structure on scaled nano-device for sensitivity enhancement and (4) to establish theoretical models and perform simulations, coupled with experimental results, for fundamental understanding.