Cyclic nucleotide gated Ca channels and non-self perception in plant pathogen defense responses

植物病原体防御反应中的环核苷酸门控 Ca 通道和非自我感知

• 批准号: 0844715
• 负责人: Gerald Berkowitz
• 金额: $ 57.07万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0844715&HistoricalAwards=false
• 关键词: Cyclic; nucleotide; gated; Ca; channels

A fundamental aspect of organism fitness involves the detection of a "non-self" presence in the extracellular environment. In the case of a pathogenic microbe invader, perception of non-self presence needs to be translated through a cellular signal transduction cascade so that the cells of the organism under attack can mount a defense or immune response. The aim of this project is to provide new insights into the early events of pathogen perception in plant cells. Prior research has shown that calcium (Ca) flow into the cytoplasm of cells through a cyclic nucleotide gated cation channel (CNGC) protein, and downstream nitric oxide (NO) generation were critical to the plant immune response signaling cascades. The research focus of this project will take advantage of our understanding of the molecular architecture related to CNGC function and regulation to elucidate further steps in this signaling cascade. Hypotheses will be tested regarding the signaling steps upstream from inward Ca conduction by this CNGC; i.e. linking pathogen perception to channel activation. Work will determine if and how cyclic nucleotide elevation occurs in plant cells responding to pathogen recognition. One component of the project aims to examine some plant cell membrane receptor proteins for possible ability to generate cyclic nucleotide, the signaling molecule that activates Ca conduction through CNGCs. Other studies will focus on identifying regulatory molecules that may impact activation of the CNGC channels, and therefore modulate plant immune response to pathogens. Broader impacts. Project activity includes use of an innovative molecular genetics teacher-training program for outreach and education efforts. This two-week full day program trains participants in molecular genetics experimental methods and underlying principles in an integrated fashion. Participants are trained, and provided with a package of tools and resources (informational and biological) to allow them to present a molecular biology experiential lab educational program in high school biology classes. This program includes a package (developed for use as part of this project) of PowerPoint presentations, protein modeling software, lab lesson plans, teacher's guides, an 80-page molecular genetics lab manual, an annotated catalog of web resources to assist high school teachers in obtaining biological and informational resources, and more. As part of the proposed broader impacts of the project, the two-week training program based on this material will be delivered to five undergraduate School of Education students as well as five current high school biology teachers each summer during the funding period. Assistance will be provided to high school teachers as they implement the program in schools throughout the State. All of the information in the teacher-training program and high school lab curriculum will be posted on a URL and made available to a wide audience of high school biology teachers through this and other web sites. The educational component of the project includes continued training of a current female African-American graduate student who will undertake research objectives of the project as part of her Ph.D. degree.

生物体适应性的一个基本方面涉及检测细胞外环境中的“非自我”存在。在病原微生物入侵者的情况下，对非自我存在的感知需要通过细胞信号转导级联进行翻译，以便受到攻击的生物体细胞可以进行防御或免疫反应。该项目的目的是为植物细胞中病原体感知的早期事件提供新的见解。先前的研究表明，钙（Ca）通过环核苷酸门控阳离子通道（CNGC）蛋白流入细胞质，下游一氧化氮（NO）的产生对植物免疫应答信号级联至关重要。该项目的研究重点将利用我们对CNGC功能和调控相关分子结构的理解来阐明该信号级联的进一步步骤。将测试关于通过该CNGC的内向Ca传导上游的信号步骤的假设;即，将病原体感知与通道激活联系起来。这项工作将确定植物细胞对病原体识别的反应是否以及如何发生环核苷酸升高。该项目的一个组成部分旨在检查一些植物细胞膜受体蛋白可能产生环核苷酸的能力，环核苷酸是通过CNGC激活Ca传导的信号分子。其他研究将集中在确定可能影响CNGC通道激活的调节分子，从而调节植物对病原体的免疫反应。更广泛的影响。项目活动包括利用创新的分子遗传学教师培训计划进行推广和教育工作。这个为期两周的全天课程以综合的方式培训参与者分子遗传学实验方法和基本原理。参与者接受培训，并提供一套工具和资源（信息和生物），使他们能够在高中生物课上展示分子生物学实验室教育计划。该计划包括PowerPoint演示文稿，蛋白质建模软件，实验室课程计划，教师指南，80页的分子遗传学实验室手册，网络资源的注释目录，以帮助高中教师获得生物和信息资源，等等。作为该项目拟议的更广泛影响的一部分，基于此材料的为期两周的培训计划将在资助期间每年夏天向五名本科教育学院的学生以及五名现任高中生物教师提供。将向高中教师提供援助，因为他们在全州的学校实施该方案。教师培训计划和高中实验室课程中的所有信息都将张贴在一个URL上，并通过这个网站和其他网站向广大高中生物教师提供。该项目的教育部分包括继续培训一名非裔美国女研究生，她将在攻读博士学位时承担该项目的研究目标。℃下