Materials World Network: Probing in-Vitro Structure-Property-Function Relationships of Ciruses at High-Resolution using Advanced Atomic Force Microscopy Methods

材料世界网络：使用先进原子力显微镜方法以高分辨率探测 Ciruses 的体外结构-性质-功能关系

• 批准号: 1008189
• 负责人: Arvind Raman
• 金额: $ 31.98万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2014-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1008189&HistoricalAwards=false
• 关键词: Materials; World; Network; Probing; Vitro

This Materials World Network project brings together Purdue University and the Universidad Autonoma de Madrid, Spain, to develop advanced Atomic Force Microscopy (AFM) techniques for investigating at high-resolution the material properties of viruses in relevant saline solutions. The goal is to enable significant advances in understanding viral function or its mitigation in medicine or in the synthesis of a new generation of nanomaterials using viruses for medicine, nanocomposites, sensors, and nanoelectronics. The proposed AFM techniques use novel multi-frequency techniques such as multi-harmonic or bimodal AFM which can provide compositional contrast in liquids far exceeding conventional methods at much faster speeds and with much greater sensitivity. The theory component of the proposed work attempts to distil metamodels from numerical simulations of mathematical models of AFM cantilevers tapping on samples in liquids, in order to link the observed material contrast to quantitative measures of local material properties. The experimental component of the proposal will investigate the inherent heterogeneities of elastic stiffness and charge density of the capsids of bacteriophages (phi 29 and T7) and eukaryotic viruses (minute virus of mice, and adenovirus) in solution and the influence of sub-surface DNA structure on the surface elasticity and charge of fully assembled viral capsids. The goal is to systematically investigate at high resolution the connection between DNA packing and capsid structure and the electromechanical properties of the assembled virus. An improved understanding of the structure-property-function relationships of viruses would not only broadly benefit medicine and virology, but it would also be a great boon to the many emerging applications of viruses in materials science and nanotechnology.The project proposes to engage strongly with the materials and biophysics communities, AFM industries, and to the larger AFM materials community via cyber-enabled initiatives on the nanohub to ensure a wide ranging impact of the proposed work.This award is co-funded by the Division of Materials Research and the Office of International Science and Engineering.

该材料世界网络项目汇集了普渡大学和西班牙马德里自治大学，开发先进的原子力显微镜（AFM）技术，用于高分辨率研究相关盐水溶液中病毒的材料特性。其目标是在理解病毒功能或其在医学中的缓解或使用病毒合成新一代纳米材料用于医学，纳米复合材料，传感器和纳米电子学方面取得重大进展。所提出的AFM技术使用新颖的多频技术，如多谐波或双峰AFM，其可以以更快的速度和更高的灵敏度提供远远超过传统方法的液体成分对比度。所提出的工作的理论部分试图从AFM杠杆在液体中的样品上敲击的数学模型的数值模拟中建立元模型，以便将所观察到的材料对比度与局部材料特性的定量测量联系起来。该提案的实验部分将研究溶液中噬菌体（phi 29和T7）和真核病毒（小鼠微小病毒和腺病毒）衣壳的弹性刚度和电荷密度的固有不均匀性，以及表面下DNA结构对完全组装的病毒衣壳的表面弹性和电荷的影响。目的是系统地调查在高分辨率的DNA包装和衣壳结构和组装的病毒的机电性能之间的连接。提高对病毒结构-性质-功能关系的理解不仅将广泛造福于医学和病毒学，而且也将对病毒在材料科学和纳米技术中的许多新兴应用带来巨大的布恩。该项目建议与材料和生物物理学社区、AFM行业、并通过nanohub上的网络支持倡议向更大的AFM材料社区提供支持，以确保拟议工作的广泛影响。由材料研究部和国际科学与工程办公室资助。