MRI: Acquisition of an LC/MSn

MRI：采集 LC/MSn

• 批准号: 1039784
• 负责人: Ralph Mead
• 金额: $ 18.79万
• 依托单位: University of North Carolina at Wilmington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-10-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1039784&HistoricalAwards=false
• 关键词: MRI; Acquisition; LC; MSn

With this award from the Major Research Instrumentation (MRI) program, Professor Ralph N. Mead and colleague Pamela J. Seaton from the University of North Carolina Wilmington will acquire a liquid chromatograph/mass spectrometer with capabilities for mass spectrometry to the nth degree (LC/MSn). The instrument will be used in a variety of research projects including characterization of photoproducts generated from the destruction of emerging contaminates in natural waters, acyl chain characterization of bacterial lipids, characterization of fossil fuel derived organic matter, binding of DNA methylating agents, structure elucidation of microbial antioxidants from hog waste, and characterization of brevetoxin photoproducts. An LC-MS combines the physical separation capability of liquid chromatography with the mass analysis ability of mass spectrometry. The liquid chromatograph separates a mixture into its molecular components. These components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with MSn capability provides additional structural identification power through further fragmentation of ions produced in the spectrometer. The instrumentation provided by this award will provide faculty and students in several departments the opportunity to pursue research projects using modern instrumentation not heretofore available at the institution. It will also be used in several laboratory courses to train significant numbers of students in the use of this important analytical technique.

北卡罗来纳大学威尔明顿分校的拉尔夫·N·米德教授和他的同事帕梅拉·J·西顿获得了主要研究仪器(MRI)项目颁发的这一奖项，他们将获得一台具有n级(LC/MSN)质谱分析能力的液体色谱仪/质谱仪。该仪器将用于各种研究项目，包括对自然水域中新出现的污染物的破坏产生的光产物的表征，细菌脂类的酰链表征，化石燃料衍生的有机物的表征，DNA甲基化试剂的结合，从生猪粪便中提取的微生物抗氧化剂的结构阐明，以及短杆菌毒素光产物的表征。LC-MS结合了液相色谱的物理分离能力和质谱学的质量分析能力。液体色谱仪将混合物分离成其分子组分。这些组分流入质谱仪，在那里它们被电离成母离子，并测量其碎片离子及其质量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。具有MSN功能的仪器通过进一步碎裂光谱仪中产生的离子来提供额外的结构识别能力。该奖项提供的仪器将为几个系的教职员工和学生提供利用该机构迄今尚未提供的现代仪器进行研究项目的机会。它还将用于几门实验室课程，以培训大量学生使用这一重要的分析技术。