MRI: Acquisition of an Orbitrap Mass Spectrometer (MS) to Accelerate Studies on Small Molecules and Broaden MS Use at UCSC

MRI：购买 Orbitrap 质谱仪 (MS) 以加速小分子研究并扩大 UCSC 的 MS 使用范围

• 批准号: 1427922
• 负责人: Phillip Crews
• 金额: $ 33.55万
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1427922&HistoricalAwards=false
• 关键词: MRI; Acquisition; Orbitrap; Mass; Spectrometer

With this award from the Major Research Instrumentation Program (MRI) and support from the Chemistry Research Instrumentation Program (CRIF), Professor Phillip Crews from the University of California Santa Cruz and colleagues Rebecca Braslau, Theodore Holman, Roger Linington and Scott Lokey will acquire an orbitrap mass spectrometer. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. In an orbitrap instrument there an outer section that acts as an electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the inner electrode. An image of the current is produced and converted to a mass spectrum using mathematical (Fourier transform) methods. This spectrometer will also be used in curricular activities involving students, many of whom are from underrepresented groups.The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) studying metabolites and natural product identification; (b) metabolomics; (c) lipidomics; and (d) organic polymer profiling.

有了这项来自主要研究仪器计划（MRI）的奖项和化学研究仪器计划（CRIF）的支持，来自加州圣克鲁斯大学的菲利普克鲁斯教授及其同事丽贝卡·布拉斯劳、西奥多霍尔曼、罗杰·利宁顿和斯科特·洛基将获得一台轨道阱质谱仪。一般来说，质谱（MS）是用于识别和表征嵌入复杂基质中的少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离成母离子及其碎片离子，并测量它们的质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。在轨道阱仪器中，有一个充当电极的外部部分和一个同轴的内部纺锤形电极，该电极捕获围绕内部电极轨道运动的离子。产生电流的图像，并使用数学（傅里叶变换）方法将其转换为质谱。该光谱仪还将用于涉及学生的课程活动，其中许多学生来自代表性不足的群体。该提案旨在加强各级的研究和教育，特别是在以下领域：（a）研究代谢物和天然产品鉴定;（B）代谢组学;（c）脂质组学;（d）有机聚合物概况分析。