MRI: Acquisition of a Liquid Chromatograph-Mass Spectrometer to Support Undergraduate Research

凭借主要研究仪器（MRI）和化学研究仪器和设施（CRIF）计划的这一奖项，印第安纳州州立大学将获得一台超高压液相色谱仪，该色谱仪与能够进行数据依赖串联质谱的线性离子阱质谱仪连接。该系统将用于分离和分析从各种来源获得的物质混合物的成分，包括从化学反应，能源相关研究和用于储能的电池中获得的样品。在该仪器中，允许液体样品通过填充有与样品组分不同程度地相互作用的物质的柱，因此组分以不同的速度移动通过柱。该过程允许组分的分离。然后使用质谱仪分析这些物质，其中组分被电离，并且通过测量离子的质荷比（m/z）来确定它们的质量。这是一种广泛使用的分析工具，用于确定混合物或材料的成分。学生将接受培训，使用这种现代仪器，而在他们的研究工作，为他们以后的职业生涯做好准备。该仪器将使新的本科实验室和研究经验。它还将用于夏季本科生研究经验（SURE）计划，该计划吸引了大量跨学科的本科生。该仪器除了为ISU的多个部门服务外，还将为包括圣玛丽伍兹学院在内的邻近机构的用户提供支持。该仪器将用于研究领域，特别是（a）神经活性天然产物的分离、合成和结构解析;（B）分析sceloporus蜥蜴的多模态信号系统;（c）碳质化石中有机物和挥发性微量元素的相关分析;（d）碳质化石中有机物和挥发性微量元素的相关分析;（e）碳质化石中有机物和挥发性微量元素的相关分析。（d）进行植物和真菌酪氨酸酶的裂解分析;和（e）进行气相离子分子化学的应用。

With this award from the Major Research Instrumentation (MRI) and Chemistry Research Instrumentation and Facilities (CRIF) programs, Indiana State University will acquire an ultra-high pressure liquid chromatograph interfaced with a linear ion trap mass spectrometer capable of data-dependent tandem mass spectrometery. The system will be used to separate and analyze the composition of mixtures of substances obtained from various sources including samples obtained from chemical reactions, energy-related research and batteries used for energy storage. In this instrument the liquid samples are allowed to pass through columns filled with substances that interact to various degrees with the sample components and thus the components move at different speeds through the columns. This process allows separation of the components. These species are then analyzed using the mass spectrometer in which the components are ionized and their masses are determined by measuring the mass to charge ratio (m/z) of the ions. This is a widely used analytical tool to determine the composition of a mixture or material. Students will be trained to use this modern instrumentation while working in their research, preparing them for their later careers. The instrument will enable new undergraduate laboratory and research experiences. It will also be used in the Summer Undergraduate Research Experiences (SURE) program which engages a large number of undergraduate students across the sciences. Besides serving multiple departments at ISU, it will support users from neighboring institutions including St. Mary-of-the-Woods College.The instrument will be used in research especially in areas such as (a) isolating, synthesizing and structural elucidating neuroactive natural products; (b) analyzing multimodal signaling systems in sceloporus lizards; (c) correlating analysis of organics and volatile trace elements in carbonaceous chondrites; (d) carrying out cleavage analysis of plant and fungal tyrosinases; and (e) carrying out applications of gas-phase ion molecule chemistry.