MRI: Acquisition of a Liquid Chromatograph-Mass Spectrometer to Support Undergraduate Research

MRI：购买液相色谱-质谱仪以支持本科生研究

• 批准号: 1531972
• 负责人: Richard Fitch
• 金额: $ 23.69万
• 依托单位: Indiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531972&HistoricalAwards=false
• 关键词: MRI; Acquisition; Liquid; Chromatograph; Mass

With this award from the Major Research Instrumentation (MRI) and Chemistry Research Instrumentation and Facilities (CRIF) programs, Indiana State University will acquire an ultra-high pressure liquid chromatograph interfaced with a linear ion trap mass spectrometer capable of data-dependent tandem mass spectrometery. The system will be used to separate and analyze the composition of mixtures of substances obtained from various sources including samples obtained from chemical reactions, energy-related research and batteries used for energy storage. In this instrument the liquid samples are allowed to pass through columns filled with substances that interact to various degrees with the sample components and thus the components move at different speeds through the columns. This process allows separation of the components. These species are then analyzed using the mass spectrometer in which the components are ionized and their masses are determined by measuring the mass to charge ratio (m/z) of the ions. This is a widely used analytical tool to determine the composition of a mixture or material. Students will be trained to use this modern instrumentation while working in their research, preparing them for their later careers. The instrument will enable new undergraduate laboratory and research experiences. It will also be used in the Summer Undergraduate Research Experiences (SURE) program which engages a large number of undergraduate students across the sciences. Besides serving multiple departments at ISU, it will support users from neighboring institutions including St. Mary-of-the-Woods College.The instrument will be used in research especially in areas such as (a) isolating, synthesizing and structural elucidating neuroactive natural products; (b) analyzing multimodal signaling systems in sceloporus lizards; (c) correlating analysis of organics and volatile trace elements in carbonaceous chondrites; (d) carrying out cleavage analysis of plant and fungal tyrosinases; and (e) carrying out applications of gas-phase ion molecule chemistry.

从主要研究仪器(MRI)和化学研究仪器和设施(CRIF)计划获得这一奖项后，印第安纳州立大学将获得一台与线性离子陷阱质谱仪接口的超高压液体色谱仪，该仪器能够进行数据相关串联质谱仪。该系统将用于分离和分析从各种来源获得的物质混合物的组成，包括从化学反应、能源相关研究和用于储能的电池获得的样品。在该仪器中，液体样品被允许通过充满物质的柱，这些物质与样品组分在不同程度上相互作用，因此组分以不同的速度通过柱。该过程允许分离各组分。然后使用质谱仪对这些物种进行分析，在质谱仪中，这些成分被电离，并通过测量离子的质量与电荷比(m/z)来确定它们的质量。这是一种广泛使用的分析工具，用于确定混合物或材料的组成。学生将接受培训，在从事研究工作的同时使用这种现代仪器，为以后的职业生涯做好准备。该仪器将为本科生提供新的实验室和研究体验。它还将用于夏季本科生研究体验(SURE)项目，该项目吸引了大量理科本科生。除了为ISU的多个部门提供服务外，它还将支持包括圣玛丽-伍兹学院在内的邻近机构的用户。该仪器将用于研究，特别是在以下领域：(A)分离、合成和结构阐明神经活性天然产物；(B)分析Scelopus蜥蜴的多模式信号系统；(C)碳质球粒陨石中有机物和挥发性微量元素的相关分析；(D)对植物和真菌酪氨酸酶进行裂解分析；以及(E)开展气相离子分子化学的应用。