MRI: Acquisition of a Liquid Chromatograph-Mass Spectrometer to Support Undergraduate Research

根据主要研究仪器（MRI）和化学研究仪器和设施（CRIF）项目的这份合同，印第安纳州立大学将获得一台超高压液相色谱仪和一台能够进行数据依赖串联质谱分析的线性离子阱质谱仪。该系统将用于分离和分析从各种来源获得的物质混合物的组成，包括从化学反应、能源相关研究和用于储能的电池中获得的样品。在该仪器中，液体样品被允许通过充满与样品组分有不同程度相互作用的物质的柱，从而使组分以不同的速度通过柱。这个过程允许组件分离。然后使用质谱仪对这些物质进行分析，其中的成分被电离，它们的质量是通过测量离子的质量与电荷比（m/z）来确定的。这是一种广泛使用的分析工具，用于确定混合物或材料的成分。学生将接受培训，在他们的研究工作中使用这种现代仪器，为他们以后的职业生涯做好准备。该仪器将为本科生提供新的实验室和研究经验。它也将用于夏季本科生研究经验（SURE）计划，该计划吸引了大量的本科生参与各个科学领域。除了为ISU的多个部门提供服务外，它还将为包括圣玛丽森林学院在内的邻近机构的用户提供支持。该仪器将用于研究，特别是在以下领域：(a)分离、合成和结构阐明神经活性天然产物；(b)分析棘孔蜥的多模态信号系统；(c)碳质球粒陨石中有机物和挥发性微量元素的对比分析；(d)对植物和真菌酪氨酸酶进行裂解分析；(e)开展气相离子分子化学的应用。

With this award from the Major Research Instrumentation (MRI) and Chemistry Research Instrumentation and Facilities (CRIF) programs, Indiana State University will acquire an ultra-high pressure liquid chromatograph interfaced with a linear ion trap mass spectrometer capable of data-dependent tandem mass spectrometery. The system will be used to separate and analyze the composition of mixtures of substances obtained from various sources including samples obtained from chemical reactions, energy-related research and batteries used for energy storage. In this instrument the liquid samples are allowed to pass through columns filled with substances that interact to various degrees with the sample components and thus the components move at different speeds through the columns. This process allows separation of the components. These species are then analyzed using the mass spectrometer in which the components are ionized and their masses are determined by measuring the mass to charge ratio (m/z) of the ions. This is a widely used analytical tool to determine the composition of a mixture or material. Students will be trained to use this modern instrumentation while working in their research, preparing them for their later careers. The instrument will enable new undergraduate laboratory and research experiences. It will also be used in the Summer Undergraduate Research Experiences (SURE) program which engages a large number of undergraduate students across the sciences. Besides serving multiple departments at ISU, it will support users from neighboring institutions including St. Mary-of-the-Woods College.The instrument will be used in research especially in areas such as (a) isolating, synthesizing and structural elucidating neuroactive natural products; (b) analyzing multimodal signaling systems in sceloporus lizards; (c) correlating analysis of organics and volatile trace elements in carbonaceous chondrites; (d) carrying out cleavage analysis of plant and fungal tyrosinases; and (e) carrying out applications of gas-phase ion molecule chemistry.