MRI: Acquisition of Liquid Chromatograph/Mass Spectrometer for Research and Education

该奖项由重大研究仪器(MRI)和化学研究仪器和设施(CRIF)计划支持。来自北伊利诺伊大学的Victor Ryzhov教授和他的同事Ralph Wheeler、Elizabeth Gaillard、Melissa Lenczewski和James Horn正在获得一台液体色谱仪、离子陷阱、飞行时间质谱仪(LC-IT-TOF-MS)。通常，质谱仪是用于识别和表征复杂样品中的少量化学物种的关键分析方法之一。在一个典型的实验中，这些成分流入质谱仪，在那里它们被电离并进行测量。这种高度灵敏的技术允许检测和确定复杂混合物中的分子结构。在带有离子捕获器的仪器中，磁场和电场的组合捕捉带电粒子。有了飞行时间能力，样品被精细地蒸发成喷雾，然后离子被电场加速，以进行进一步的表征。该液相色谱部件允许在分析组分之前分离混合物。这项收购加强了该大学和地区的研究基础设施。该仪器通过让不同的学生参与到使用这种现代分析技术的研究和培训中来扩大参与。该仪器服务于各种本科院校的学生和研究人员，如哈珀学院、岩谷学院、诺克斯学院以及橄榄拿撒勒大学。这种质谱仪增强了各级研究和教育。该工具有利于研究可能与眼睛相关的工程蛋白质和年龄相关过程的研究人员。该仪器还用于可能与理解蛋白质组学、开发用于硼中子捕获的递送剂和发现酶抑制剂相关的研究。质谱计还可以研究复杂有机合成的新合成方法；这些方法可能与药物发现研究相关。最后，利用该仪器对多肽光降解和水污染物进行了研究。

This award is supported by the Major Research Instrumentation (MRI) and Chemical Research Instrumentation and Facilities (CRIF) Programs. Professor Victor Ryzhov from Northern Illinois University and colleagues Ralph Wheeler, Elizabeth Gaillard, Melissa Lenczewski and James Horn are acquiring a liquid chromatograph, ion-trap, time-of-flight mass spectrometer (LC-IT-TOF-MS). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex samples. In a typical experiment, the components flow into a mass spectrometer where they are ionized and measured. This highly sensitive technique allows the detection and determination of the structures of molecules in a complex mixture. In an instrument with an ion trap, a combination of magnetic and electric fields capture the charged particles. With the TOF capabilities the sample is finely vaporized into a spray, then the ions are accelerated by an electric field to allow further characterization. The liquid chromatograph component allows separation of mixtures before the components are analyzed. This acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse students in research and training using this modern analytical technique. The instrument serves students and researchers from various undergraduate colleges such as Harper, Rock Valley and Knox Colleges as well as Olivet Nazarene University.This mass spectrometer enhances research and education at all levels. The tool benefits researchers studying engineering proteins and age-related processes that could be relevant to the eye. The instrumentation is also used for studies that may be relevant for understanding proteomics, developing delivery agents for boron neutron capture, and discovering enzyme inhibitors. The mass spectrometer also enables research for new synthetic methods for complex organic synthesis; These methods that may be relevant for drug discovery studies. Finally, the instrumentation is used to study peptide photodegradation and water pollutants.