Analysis of uniparental elimination of chromosomes in wide crosses

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Complete uniparental chromosome elimination occurs in some interspecific hybrids of plants. The aim of this project is to investigate the processes of selective elimination of paternal chromosomes during the development of hybrid embryos and to establish whether the highly conserved histone H3 like centromeric protein A (CENH3) acts as a regulator of the mitosis-dependent elimination process. In addition, genes involved in the mitosis-independent chromosome elimination process will be identified. Different types of H. vulgare x H. bulbosum crosses will be used as a model, because in this species combination the degree of chromosome retention is influenced by the genotype of both parents as well as environmental conditions after fertilization. Besides a better understanding on interaction of parental genomes in newly formed hybrids, the gained knowledge could result in establishing efficient methods for generating of haploids or new species combinations.