Analysis of uniparental elimination of chromosomes in wide crosses

宽杂交中染色体单亲消除分析

• 批准号: 40013986
• 负责人: Privatdozent Dr. Andreas Houben
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/40013986?language=en
• 关键词: Analysis; uniparental; elimination; chromosomes; wide

Complete uniparental chromosome elimination occurs in some interspecific hybrids of plants. The aim of this project is to investigate the processes of selective elimination of paternal chromosomes during the development of hybrid embryos and to establish whether the highly conserved histone H3 like centromeric protein A (CENH3) acts as a regulator of the mitosis-dependent elimination process. In addition, genes involved in the mitosis-independent chromosome elimination process will be identified. Different types of H. vulgare x H. bulbosum crosses will be used as a model, because in this species combination the degree of chromosome retention is influenced by the genotype of both parents as well as environmental conditions after fertilization. Besides a better understanding on interaction of parental genomes in newly formed hybrids, the gained knowledge could result in establishing efficient methods for generating of haploids or new species combinations.

在一些植物的种间杂交中，单亲染色体完全消除。本项目的目的是研究杂交胚胎发育过程中父本染色体的选择性消除过程，并确定高度保守的组蛋白H3类似着丝粒蛋白A(CENH3)是否在依赖有丝分裂的消除过程中发挥调节作用。此外，还将识别与有丝分裂无关的染色体消除过程中涉及的基因。不同类型的球茎海棠杂交组合将被用作模式，因为在这个物种组合中，染色体保留的程度受到双亲的基因型以及受精后的环境条件的影响。除了更好地了解亲本基因组在新形成的杂种中的相互作用外，所获得的知识还可能导致建立有效的方法来产生单倍体或新的物种组合。