Luzula elegans - a holocentric species with an inverted sequence of meiotic events?

Luzula elegans - 具有倒置减数分裂事件序列的全中心物种？

• 批准号: 216463552
• 负责人: Privatdozent Dr. Andreas Houben
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/216463552?language=en
• 关键词: Luzula; elegans; holocentric; species; inverted

Meiosis in organisms with holocentric chromosomes illustrates that our views of meiotic chromosome arrangement and control of meiosis based on observations of monocentric chromosomes may not apply to all organisms. To proof whether in the holocentric genus Luzula the sequence of meiotic events is inverted (first division is equational whereas the second division is reductional), the project aims to investigate the sequence of meiotic events in L. elegans using advanced high-resolution microscopy. L. elegans genes encoding proteins involved in meiotic recombination, crossover formation and sister chromatid cohesion/separation will be identified and their sequence information will be used to generate antibodies for localisation of these proteins on meiotic chromosomes. In particular, the chromatin domain responsible for the end-to-end association of homologous (non-sister) chromatids during meiosis I - II transition and the type of alignment between homologous chromatids in meiosis II will be analysed. Chromosome-specific FISH marker will be used to trace the dynamics of individual chromosomes during meiosis.

具有全着丝粒染色体的生物体中的减数分裂表明，我们基于对单着丝粒染色体的观察对减数分裂染色体排列和减数分裂控制的看法可能并不适用于所有生物体。为了证明全中心线虫属的减数分裂事件序列是否是倒置的（第一次分裂是等式分裂，而第二次分裂是还原性分裂），该项目旨在使用先进的高分辨率显微镜研究线虫减数分裂事件的序列。编码参与减数分裂重组、交叉形成和姐妹染色单体凝聚/分离的蛋白质的线虫基因将被鉴定，并且它们的序列信息将用于生成用于在减数分裂染色体上定位这些蛋白质的抗体。特别是，将分析减数分裂 I - II 过渡期间负责同源（非姐妹）染色单体端到端关联的染色质结构域以及减数分裂 II 中同源染色单体之间的比对类型。染色体特异性 FISH 标记将用于追踪减数分裂过程中单个染色体的动态。