Luzula elegans - a holocentric species with an inverted sequence of meiotic events?

具有全着丝点染色体的生物的减数分裂表明，我们关于减数分裂染色体排列和减数分裂控制的观点基于对单着丝点染色体的观察，可能并不适用于所有生物。为了证明Luzula属的减数分裂事件序列是否是颠倒的(第一次分裂是等式的，第二次分裂是还原的)，该项目旨在利用先进的高分辨率显微镜研究线虫的减数分裂事件序列。将鉴定编码参与减数分裂重组、交叉形成和姐妹染色单体聚集/分离的蛋白质的基因，并利用它们的序列信息产生抗体，用于定位这些蛋白质在减数分裂染色体上的位置。特别是，负责减数分裂I-II转换期间同源(非姊妹)染色单体端到端关联的染色质结构域以及减数分裂II中同源染色单体之间的排列类型将被分析。染色体特异的FISH标记将被用来追踪减数分裂过程中单个染色体的动态。

Meiosis in organisms with holocentric chromosomes illustrates that our views of meiotic chromosome arrangement and control of meiosis based on observations of monocentric chromosomes may not apply to all organisms. To proof whether in the holocentric genus Luzula the sequence of meiotic events is inverted (first division is equational whereas the second division is reductional), the project aims to investigate the sequence of meiotic events in L. elegans using advanced high-resolution microscopy. L. elegans genes encoding proteins involved in meiotic recombination, crossover formation and sister chromatid cohesion/separation will be identified and their sequence information will be used to generate antibodies for localisation of these proteins on meiotic chromosomes. In particular, the chromatin domain responsible for the end-to-end association of homologous (non-sister) chromatids during meiosis I - II transition and the type of alignment between homologous chromatids in meiosis II will be analysed. Chromosome-specific FISH marker will be used to trace the dynamics of individual chromosomes during meiosis.