Targeted engineering of plant centromeres

植物着丝粒的靶向工程

• 批准号: 244949195
• 负责人: Privatdozent Dr. Andreas Houben
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/244949195?language=en
• 关键词: Targeted; engineering; plant; centromeres

Engineered minichromosomes are highly desirable tools for analysis of plant chromosome function, biotechnology and plant breeding purposes. In preliminary studies, we have developed a method for the de novo generation of centromeres via the targeting of recombinant kinetochore proteins to tandem repeat arrays at non-centromeric positions and established a method for non-targeted chromosome truncation via telomere seeding in plants. Now, we aim to combine both methods for the generation of engineered minichromosomes. For this, targeted de novo centromere and targeted chromosome truncation will be applied as an experimental system in A. thaliana. Further, kinetochore assembly factors and the epigenetic setup which are required for efficient de novo centromere formation will be deciphered. The mitotic and meiotic activity of de novo centromeres will be determined.

工程微染色体是植物染色体功能分析、生物技术和植物育种的理想工具。在初步研究中，我们已经开发了一种方法，从头生成的着丝粒通过靶向重组动粒蛋白的串联重复阵列在非着丝粒位置，并建立了一种方法，非靶向染色体截短通过端粒播种植物。现在，我们的目标是将这两种方法联合收割机用于产生工程化的微型染色体。为此，将定向从头着丝粒和定向染色体截短作为实验系统应用于A. thaliana.此外，着丝粒组装因子和高效从头着丝粒形成所需的表观遗传设置将被破译。将测定从头着丝粒的有丝分裂和减数分裂活性。

项目成果

Engineering of plant chromosomes

• DOI: 10.1007/s10577-014-9449-1
• 发表时间: 2015-01
• 期刊: Chromosome Research
• 影响因子: 2.6
• 作者: M. Mette;A. Houben