Analysis of B chromosome transcripts and their involvement in B chromosome nondisjunction

B 染色体转录物分析及其与 B 染色体不分离的关系

• 批准号: 59593897
• 负责人: Privatdozent Dr. Andreas Houben
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/59593897?language=en
• 关键词: Analysis; chromosome; transcripts; their; involvement

Drive is the main feature of B chromosomes (Bs) in certain species of plants, especially the Gramineae, where it involves directed nondisjunction at pollen mitosis and allows the Bs to be transmitted at greater rate than Mendelian frequencies. Thus far however no gene, or nondisjunction element, on any B has yet been characterized that plays a role in B accumulation mechanisms/drive. We wish to test whether noncoding RNA is involved in the regulation of B nondisjunction. To this end Bspecific transcriptionally active high-copy repeats, which are located at the Bterminal regions (the chromosomal region which controls the process of nondisjunction), will be analyzed, together with with the hitherto uncharacterized Bspecific transcripts. In addition, we intend to analyse the process of nondisjunction at the microscopic level, in terms of differences in the chromatin composition, and centromere function between A and B chromosomes. This analysis will provide new evidence of the generation of rapid genome changes in higher eukaryotes, with particular relevance to selfish chromosomes, and will improve our knowledge of the mechanism underlying the segregation behaviour of chromosomes.

在某些植物中，特别是在禾本科植物中，驱动是B染色体（Bs）的主要特征，其中它涉及花粉有丝分裂的定向不分离，并且允许Bs以比孟德尔频率更高的速率传递。然而，迄今为止，还没有任何B上的基因或不分离元件被表征为在B积累机制/驱动中起作用。我们希望测试非编码RNA是否参与B不分离的调节。为此，将分析位于B末端区域（控制不分离过程的染色体区域）的B特异性转录活性高拷贝重复序列，以及迄今为止未表征的B特异性转录物。此外，我们打算分析的过程中不分离在微观水平上，在染色质组成的差异，和A和B染色体之间的着丝粒功能。这种分析将提供新的证据，在高等真核生物的快速基因组变化的一代，特别是自私的染色体，并将提高我们的知识的分离行为的机制。