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Sizing the genetic and physical centromere of barley chromosomes as a prerequisite of understanding genome evolution in the genus Hordeum

测定大麦染色体的遗传和物理着丝粒大小是了解大麦属基因组进化的先决条件

基本信息

批准号：
242298047
负责人：
Privatdozent Dr. Andreas Houben
金额：
--
依托单位：
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/242298047?language=en
关键词：
Sizing genetic physical centromere barley

项目摘要

Entire genome characterization of the important crop species barley requires knowledge of the correlation between the genetic and physical maps. Genetic map distances are based on recombination values and can not be linearly translated into actual physical distances of molecular markers or genes. In regions with suppressed recombination, i.e. the genetic centromeres and pericentromeric regions of barley chromosomes, genetic resolution is low and short genetic distances between markers may translate to long physical distances. Here the order of gene/marker loci can be resolved by physical or cytological mapping. In order to improve the resolution of the physical map of barley in the (peri)centromeric region of chromosome 3H we intend to use fluorescence in situ hybridization (FISH) to resolve the physical linear order of 100 repeat-free single-copy sequences, which were genetically assigned to a 10cM bin around the centromere. The sequence composition of the centromere will be determined based on FISH-anchored BACs. Furthermore, the established order of these probes on barley chromosome 3H will be used to test for their order in related Hordeum species and to evaluate the extent of chromosome rearrangements that might have played a role in the evolution of the genus Hordeum.

大麦这一重要作物物种的全基因组表征需要了解遗传图谱和物理图谱之间的相关性。遗传图谱距离基于重组值，不能线性转化为分子标记或基因的实际物理距离。在重组受到抑制的区域，即大麦染色体的遗传着丝粒和周围着丝粒区域，遗传分辨率较低，标记之间的短遗传距离可能转化为较长的物理距离。在这里，基因/标记位点的顺序可以通过物理或细胞学定位来解决。为了提高大麦染色体3H（周围）着丝粒区物理图谱的分辨率，我们打算使用荧光原位杂交（FISH）来解析100个无重复单拷贝序列的物理线性顺序，这些序列被遗传分配到着丝粒周围10cM的bin中。着丝粒的序列组成将基于fish锚定bac来确定。此外，这些探针在大麦染色体3H上建立的顺序将用于测试它们在相关的Hordeum物种中的顺序，并评估染色体重排的程度，这些重排可能在Hordeum属的进化中发挥作用。