Analysis of the relationship between heterosis and the epigenetic modification of DNA and histones in Arabidopsis thaliana

拟南芥杂种优势与DNA和组蛋白表观遗传修饰关系分析

• 批准号: 5453246
• 负责人: Privatdozent Dr. Andreas Houben
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5453246?language=en
• 关键词: Analysis; relationship; between; heterosis; epigenetic

Arabidopsis accessions Col-0 and C24 and their reciprocal F1 hybrids exhibiting biomass heterosis are employed as a model system to investigate the complex relationship between chromatin structure and gene regulation in heterosis. To reach our goal, histone modifications and DNA methylation are being compared between parental and hybrid plants at the single gene and the genomic level. The analysis of specific genes is focused on 7 selected genes for which in expression analysis a positive or negative dominance effect has been demonstrated. The extent of epigenetic modifications at gene promoters will be compared using ChlP-PCR to quantify histone methylation and methylation-sensitive restriction cleavage followed by PCR to determine DNA methylation. Genomic level studies will be performed by Chip on chip analysis of chromosome 4 (in collaboration) and optionally by methylation-sensitive AFLP. The results are expected to identify epigenetic modifications correlated to gene regulation in heterosis and thereby will provide new insights to the importance of histone and DNA modifications in general.

采用拟南芥种质 Col-0 和 C24 及其表现出生物量杂种优势的倒数 F1 杂种作为模型系统，研究杂种优势中染色质结构与基因调控之间的复杂关系。为了实现我们的目标，我们正在单基因和基因组水平上比较亲本植物和杂交植物之间的组蛋白修饰和 DNA 甲基化。特定基因的分析集中于7个选定的基因，在表达分析中已证明这些基因具有正显性或负显性效应。将使用 ChlP-PCR 来比较基因启动子处的表观遗传修饰的程度，以量化组蛋白甲基化和甲基化敏感的限制性切割，然后使用 PCR 来确定 DNA 甲基化。基因组水平研究将通过 4 号染色体的芯片分析（合作）进行，也可以选择通过甲基化敏感的 AFLP 进行。预计这些结果将鉴定与杂种优势基因调控相关的表观遗传修饰，从而为组蛋白和 DNA 修饰的重要性提供新的见解。