新しい口腔癌マーカーエンザイムとしての血清ジペプチジルペプチダーゼIV-担癌患者血清中のTリンパ球DPPIV抑制因子について-

血清二肽基肽酶IV作为新型口腔癌标志酶-关于癌症患者血清中T淋巴细胞DPPIV抑制因子-

• 批准号: 08771912
• 负责人: 上松 隆司
• 金额: $ 0.64万
• 依托单位: Matsumoto Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08771912/
• 关键词: 血清ジペプチジルペプチダーゼIV; Tリンパ球; 口腔癌; 腫瘍マーカー; CD26

健常人と担癌患者の末梢血Tリンパ球数と血清DPPIV活性の関係を抗CD3,CD4,CD8およびCD26(DPPIV)抗体を用いてフローサイトメロリ-で検討したところ,担癌患者末梢血リンパ球数(P<0.001),Tリンパ球数(P<0.001),Tリンパ球サブセットのうちCD4(P<0.01)およびCD8陽性細胞数(P<0.05)は健常人に比べ有意に低下していた.担癌患者血清中のDPPIV活性はTリンパ球細胞膜DPPIV(CD26)抗原量と同様に健常人に比べ有意(P<0.001)に低下していた.非働化10%牛胎児血清,2-MercaptoethanolとTリンパ球刺激物質であるPHAまたはConA,Tリンパ球増殖因子であるIL-2を添加したRPMI1640培地を増殖培地とし,Lymphoprep^<TM>で分離した健常人単核球(以下PMNC)を,ポアサイズが0.45μmのメンブランで隔てた状態で癌細胞とco-cultureしたところ,PMNCのみで培養した対照に比べリンパ球のCell clump数および芽球化反応が低下(5〜25%)した.10%の健常人血清を含む増殖培地を用いた健常人リンパ球培養系において,口腔扁平上皮患者血清の割合を増加させるとリンパ球芽球化反応が低下する傾向がみられた.以上の結果より,末梢血Tリンパ球数と血清DPPIV活性の間には正の相関関係がみられること,担癌患者血清によりTリンパ球刺激物質または増殖因子によるリンパ球芽球化反応が低下することが明らかとなり,癌細胞由来の液性因子がTリンパ球の活性化とCD26(DPPIV)の発現を抑制している可能性が示唆された.

The serum DPPIV activity, anti-CD3,CD4,CD8 antibody and CD26 (DPPIV) antibody in the peripheral blood of normal patients with cancer were measured. The results were as follows: the number of peripheral blood samples of normal patients with cancer, the number of T cells in the peripheral blood of patients with cancer (P&lt). The number of CD8 sexual cells in normal people is lower than that in normal people. The level of DPPIV activity in serum of patients with burden cancer was significantly lower than that of healthy controls (P < 0.01). The amount of DPPIV (CD26) antigen on the membrane of globular membrane in patients with burden cancer was lower than that in normal people. Non-digested 10% bovine fetal serum, 2-Mercaptoethanol T serum, bulb stimulator, PHA, ConA,T, globular colonization factor, IL-2, add RPMI1640 culture, Lymphoprep^ & lt;TM&gt The nuclear spheres (PMNC) of normal people are separated from each other, and the cells of cancer cells are separated from each other. Co-culture cells of cancer cells are isolated, and PMNC cells are cultured. The results show that the number of Cell clump is lower than that of normal people. 10% of normal people's serum contains human serum, which is used in colonization culture. The serum of patients with oral flat epithelium showed spheroidization and spheroidization of spheroidization. The results of the above results showed that the serum DPPIV activity of T lymphocytes in peripheral blood was significantly higher than that in peripheral blood, and the serum levels of T lymphocytes in patients with cancer were significantly higher than those in patients with cancer. Cancer cell origin, fluid factor T, globular activation, CD26 (DPPIV), inhibition, possibility, instillation.

项目成果

Uematsu,T.: "Reduced expression of dipeptidyl peptidase (DPP) IV in peripheral blood T lymphocytes of oral cancer patients" Journal of Oral Pathology and Medicine. Vol.9. 507-512 (1996)

Uematsu,T.：“口腔癌患者外周血 T 淋巴细胞中二肽基肽酶 (DPP) IV 的表达降低”《口腔病理学与医学杂志》。