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In vivo analysis of mechanism for neuronal regeneration in damaged brain by using multi-nuclear, multi-modality MRI methods

利用多核、多模态 MRI 方法体内分析受损脑神经元再生机制

基本信息

批准号：
16591216
负责人：
NARUSE Shoji
金额：
$ 2.24万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

We have developed multi-modality magnetic resonance imaging(MRI) techniques for analysis of neuronal regeneration in damaged brain. Those are ; (1)Fiber tracking imaging method by using diffusion tensor images obtained from ultra-fast diffusion weighted imaging ; (2)Neuronal activation imaging method by using manganese-enhanced MRI(MEMRI). This method depends on the theory that Mn ion can enter into neuronal cell in conjunction with Ca ion during the neuronal activation ; (3)Two dimensional MR spectroscopy(2D-MRS) by which various brain metabolites can be analyzed precisely in damaged brain ; (4)Perfusion imaging using arterial spin tagging method, (4)Non-invasive perfusion imaging of the brain by using water signal in the vessel as a tracer(Arterial spin tagging method). MRI devices used were experimental MRI machines (4.7T Bruker Biospec and TOT Varian INOVA300SWB) and a whole body MRI machine for human(1.5T GE Signa Horizon, 1.5T Siemens Symphony and 3.0T GE Signa Horizon).In fiber … More tracking imaging method, three dimensional distribution of fiber orientation was clearly demonstrated by the images based on diffusion tensor direction. Disconnection of fiber was demonstrated in cases of cerebral infarction. In MEMRI method, neuronal fibers were clearly demonstrated in rat olfactory grove, optic nerve and optic radiation. Also the six cortical layers were clearly demonstrated by this method. This method is useful to detect the neuronal orientation and brain structure directly based on the nerve cell activation. In 2D-MRS, 2D-JPRESS and 2D-COSY were developed on 1.5T and 3.0T clinical MRI devices. Brain metabolites which could not be detected by conventional MRS method were obtained by those 2D-MRS method, such as glutamine/glutamate, glutathione, threonine and GABA. 2D-MRS is useful to examine the viability of damaged brain from the metabolic point of view. The perfusion imaging using arterial spin tagging method was useful to detect the cerebral blood flow change non-invasively, and the combination with diffusion weighted imaging is useful to know the viability of penumbra region in cerebral infarction.In conclusion, the multi-modality MR methods are unique and useful to examine the regeneration of damaged brain tissue and nerve cell function non-invasively. Since there are still more techniques in MRI methods to evaluate the brain function and metabolism, further research should be continued. Less

我们开发了多模态磁共振成像（MRI）技术，用于分析受损大脑中的神经元再生。这些是;（1）利用超快速扩散加权成像获得的扩散张量图像的纤维追踪成像方法;（2）利用锰增强MRI（MEMRI）的神经元激活成像方法。（3）二维磁共振波谱技术（2D-MRS），可准确分析损伤脑组织中各种代谢物;（4）灌注成像采用动脉自旋标记法，（4）通过使用血管中的水信号作为示踪剂的脑的非侵入性灌注成像（动脉自旋标记法）。使用的MRI设备是实验MRI机器（4.7T Bruker Biospec和TOT Varian INOVA 300 SWB）和用于人的全身MRI机器（1.5T GE Signa Horizon、1.5T Siemens Symphony和3.0T GE Signa Horizon）。 ...更多信息追踪成像方法，基于扩散张量方向的图像清晰地显示了纤维取向的三维分布。脑梗塞例出现纤维中断。MEMRI法可清晰显示大鼠嗅格罗夫、视神经和视放射线的神经纤维。该方法还能清晰显示皮质的六个层次。该方法可以直接根据神经元的激活情况来检测神经元的方向和大脑结构。2D-MRS、2D-JPRESS和2D-COSY在1.5T和3.0T临床MRI设备上开发。利用2D-MRS方法获得了常规MRS方法检测不到的脑代谢产物，如谷氨酰胺/谷氨酸、谷胱甘肽、苏氨酸和GABA。2D-MRS可从代谢角度检查受损脑的存活性。动脉自旋标记法灌注成像可无创性检测脑血流变化，结合弥散加权成像可了解脑梗死半暗带区的存活情况，多模态MR成像技术可无创性检测脑组织损伤后的再生情况和神经细胞功能。由于MRI方法在评价脑功能和代谢方面仍有较多的技术手段，有待于进一步研究。少