Development of multi-modality magnetic resonance methods for cerebral blood flow, metabolism and brain function on ultra-high magnetic field and its clinical application

超高磁场下脑血流、代谢及脑功能多模态磁共振方法开发及临床应用

• 批准号: 12670897
• 负责人: NARUSE Shoji
• 金额: $ 2.18万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670897/
• 关键词: Magnetic resonance imaging (MRI); MR spectroscopy; Chemical shift Imaging (CSI); Diffusion-weighted Imaging; Perfusion Imaging; Functional MRI (fMRI); Ultra-high magnetic field; Diffusion Tensor Imaging; 拡散強調画像

We have developed multi-modality magnetic resonance imaging (MRI) techniques for analysis of cerebral circulation, brain functions and metabolism on ultra-high magnetic field. Those are; (1) Fast ^1H chemical shift imaging (CSI) method and 2D-COSY method to detect various metabolite in the brain, (2) Diffusion-weighted imaging using ultra fast MRI (EPI-DWI), (3) Per fusion imaging using arterial spin tagging method, (4) Improvement of functional MRI (fMRI) on ultra-high field magnet and development of new fMRI method which detect nerve cell excitation. Systems used were an experimental MRI machine (7.0 T INOVA300SWB, Varian) and a whole body MRI machine for human (3.0 T, Signa Horizon, GE).In ^1H-CSI, higher signal intensity was obtained than lower magnetic field systems and the distribution of various metabolites was visualized with high spatial resolution, such as N-acetyl aspartate (NAA), glutamine/glutamate, choline, creatine, inositol, lactate and etc. The decrease of NAA in vario … More us brain disorders were observed, indicating the loss of neurons. Increased lactate was observed in hypoxic cell fractions in malignant tumors and infarction. In 2D-COSY method, glutamine and glutamate were clearly separated and small amount of ammo acid such as taurine could be detected. In the EPI-DWI, cerebral infarction is detected at an ultra-acute stage such as 30 minutes after the onset. The diffusion tensor imaging (DTT) and fiber tracking imaging, which were calculated from the data of EPI-DWI, indicated the direction of nerve fiber clearly. The perfusion imaging using arterial spin tagging method (FAIR) is useful to detect the relative changes in cerebral blood flow, but it still requires more sensitivity even on a 3.0T system. The combination of EPI-DWI and Perfusion Imaging can possibly indicate the reversibility of damaged brain tissue. In functional MRI (fMRI), an increase of signal intensity was observed on the ultra-high magnetic field in the cortical area corresponding to each activation task such as finger movements, photic stimulation and hearing words. The fMRI was also obtained by complex tasks such as imagination. As a new fMRI method, we tried to develop topographic imaging of neuronal activity by the combination with microimaging and EPI-DWI. Theoretically it is, possible to observe the influx of water molecule in the excited nerve cell. Although we could obtain the ultra-fast diffusion weighted microimage with matrices size of 10 mirometer each, it is not enough to detect the influx of nerve cell directly so far on the experimental animal. The reason would be that the gradient strength is not enough for this experiment. More strong gradient and more strong magnetic field is necessary for this experiments. Nevertheless, the multi-modality MR methods are unique and useful methods to examine the brain metabolism and function non-invasively and to examine pathophysiology of various cerebral disorders. In this sense, further research should be continued. Less

我们开发了多模态磁共振成像（MRI）技术，用于分析超高磁场下的脑循环、脑功能和代谢。这些是； （1）快速^1H化学位移成像（CSI）方法和2D-COSY方法检测大脑中的各种代谢物，（2）使用超快速MRI的扩散加权成像（EPI-DWI），（3）使用动脉自旋标记方法的每次融合成像，（4）超高磁场磁体上功能MRI（fMRI）的改进以及检测神经细胞的新fMRI方法的开发 激发。使用的系统是实验性 MRI 机器（7.0 T INOVA300SWB，Varian）和人体全身 MRI 机器（3.0 T，Signa Horizo​​n，GE）。在 ^1H-CSI 中，比低磁场系统获得更高的信号强度，并且以高空间分辨率可视化各种代谢物的分布，例如 N-乙酰天冬氨酸（NAA）、谷氨酰胺/谷氨酸、胆碱、 肌酸、肌醇、乳酸等。在各种脑部疾病中观察到NAA的减少，表明神经元的损失。在恶性肿瘤和梗塞的缺氧细胞部分中观察到乳酸增加。 2D-COSY方法中，谷氨酰胺和谷氨酸盐清晰分离，并且可以检测到少量的氨基酸，例如牛磺酸。在EPI-DWI中，脑梗塞在发病后30分钟等超急性阶段被检测到。根据EPI-DWI数据计算出的弥散张量成像（DTT）和纤维追踪成像清楚地指示了神经纤维的方向。使用动脉自旋标记方法（FAIR）的灌注成像有助于检测脑血流的相对变化，但即使在3.0T系统上仍然需要更高的灵敏度。 EPI-DWI 和灌注成像的结合可能可以指示受损脑组织的可逆性。在功能性磁共振成像（fMRI）中，在与手指运动、光刺激和听到单词等每个激活任务相对应的皮质区域的超高磁场中观察到信号强度的增加。功能磁共振成像也是通过想象等复杂任务获得的。作为一种新的功能磁共振成像方法，我们尝试结合显微成像和EPI-DWI来开发神经元活动的地形成像。理论上，可以观察到水分子在兴奋的神经细胞中的流入。虽然我们可以获得每个矩阵尺寸为10微米的超快扩散加权显微图像，但迄今为止还不足以直接检测实验动物上神经细胞的流入。原因是梯度强度对于本实验来说不够。该实验需要更强的梯度和更强的磁场。尽管如此，多模态磁共振方法是非侵入性检查大脑代谢和功能以及检查各种脑部疾病的病理生理学的独特且有用的方法。从这个意义上说，还应该继续进行进一步的研究。较少的

项目成果

Takegami T, Naruse, S. et al.: "Mismatch between lactate and the apparent diffusion coefficient of water in progressive focal ischemia"NMR in Biomedicine. 14・2. 5-11 (2001)

Takegami T，Naruse，S.等人：“进行性局部缺血中乳酸和水的表观扩散系数之间的不匹配”NMR in Biomedicine 14・2（2001）。

Naruse S., Tanaka C.: "Functional MRI (in Japanese)"Orthopaedic Traumatolog. 44. 679-685 (2001)

Naruse S.，Tanaka C.：“功能性 MRI（日语）”骨科创伤学。

Naruse S., Tanaka C.: "Principle of Diagnosti Method and its Characteristics for Diagnosis of Brain Disease ; Functional MRI (in Japanese)"Clinician. 47. 657-665 (2000)

Naruse S.，Tanaka C.：“脑部疾病诊断方法的原理及其特征；功能性 MRI（日语）”临床医生。

Fukunaga M., Tanaka C., Umeda M., Ebisu I., Aoki I., Someya Y., Watanabe Y., Mori Y., Naruse S.: "Functional MRI of the brain (inJapanese)"Jap J Magn Reson in Med.. 21(6). 204-216 (2001)

Fukunaga M.、Tanaka C.、Umeda M.、Ebisu I.、Aoki I.、Someya Y.、Watanabe Y.、Mori Y.、Naruse S.：“大脑的功能 MRI（日语）”Jap J Magn Reson

Ebisu T., Katsuta K., Fujikawa A., Aoki I., Umeda M., Naruse S., Tanaka C.: "Early and delayed neuroprotective effects of FK506 on experimental focal ischemia quantitatively assessed by diffusion-weighted MRI"Magn Reson. Imag.. 19. 153-160 (2001)

Ebisu T.、Katsuta K.、Fujikawa A.、Aoki I.、Umeda M.、Naruse S.、Tanaka C.：“通过扩散加权 MRI 定量评估 FK506 对实验性局灶性缺血的早期和延迟神经保护作用”Magn Reson