Analysis of the mechanism of neuronal regeneration by using multi-nuclei magnetic resonance methods with molecular imaging of brain function

脑功能分子影像多核磁共振方法分析神经元再生机制

• 批准号: 20591455
• 负责人: NARUSE Shoji
• 金额: $ 2.91万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591455/
• 关键词: 磁気共鳴法(MRI); 脳機能分子イメージング; Mn-enhanced MRI (MEMRI); 神経細胞再生; 2次元磁気共鳴スペクトル; 免疫; 超高速化学シフト画像; Mn-enhanced MRI

We have developed multi-nuclear magnetic resonance method with molecular imaging for the analysis of neuronal function and regeneration as followings ; (1) Neuronal activation imaging by using manganese-enhanced MRI (MEMRI) which is based on the theory that Mn2+ can enter into neuronal cell in conjunction with Ca2+ during the neuronal activation; This method could clearly demonstrate the neuronal activation of the sensory area in rat and the structure of neuronal fibers in rat olfactory grove, optic radiation and cortical layers. During the recovery period after experimental ischemia, the enhanced area by MEMRI corresponded to the GFAP-positive astroglia were observed in the peripheral region of the ischemic core, indicating the reactive astrogliosis. (2) Magnetic resonance technique which can visualize antigen-antibody reaction in the brain: The imaging of macromolecule combined with magnetic resonance sensitive materials (Fe, Gd) could be obtained in vitro, but it was difficult to apply this method to in vivo study. (3) We have developed 2D-MRS method on the clinical MRI scanner by using 2D-JPRESS and 2D-COSY methods. Brain metabolites were detected sensitively by 2D-MRS method. The multi-nuclear MR methods are useful to examine the brain function and neuronal regeneration non-invasively.

本研究建立了多核磁共振分子成像技术用于神经元功能和再生的研究：（1）神经元激活的锰增强磁共振成像（MEMRI），它是基于神经元激活过程中Mn ~（2+）与Ca ~（2+）共同进入神经元的理论;该方法能清楚地显示大鼠感觉区神经元的活动以及嗅格罗夫层、视辐射层和皮层神经元纤维的结构。实验性脑缺血后恢复期，MEMRI显示缺血核心区周围出现GFAP阳性星形胶质细胞的增强区，提示反应性星形胶质细胞增生。(2)磁共振成像技术可显示脑内抗原抗体反应：大分子与磁共振敏感材料（Fe、Gd）的结合可在体外成像，但难以应用于体内研究。(3)我们利用2D-JPRESS和2D-COSY方法在临床MRI扫描仪上开发了2D-MRS方法。2D-MRS方法敏感地检测脑代谢物。多核磁共振成像技术可以无创地检测脑功能和神经元再生。

项目成果

Correction of Eddy Currents for Time-domain-interleaved Blipped-phase -encoding Echo-planar Spectroscopic Imaging.

时域交错 Blipped 相位编码回波平面光谱成像的涡流校正。

• 发表时间: 2010
• 作者: Bito Y;Hirata K;Ebisu T;Kawai Y;Tanaka C;et al.
• 通讯作者: et al.

A new imaging of muscle deformation applied electrical stimulation using MRI

使用 MRI 进行电刺激的肌肉变形新成像

• 发表时间: 2009
• 作者: Watanabe Y;Umeda M;Higuchi T;Tanaka C;et al
• 通讯作者: et al

Diffusion-Weighted Line-Scan Echo-Planar Spectroscopic Imaging for Improved Accuracy in Metabolite Diffusion Imaging.

扩散加权线扫描回波平面光谱成像可提高代谢物扩散成像的准确性。

• 发表时间: 2009
• 作者: Kimura K;Watanabe Y;Umeda M;Tanaka C;et al
• 通讯作者: et al

In vivo visualization of reactive gliosis using manganese-enhanced magnetic resonance imaging.

使用锰增强的磁共振成像对反应性神经胶质病的体内可视化。

• DOI: 10.1016/j.neuroimage.2009.11.005
• 发表时间: 2010-02-15
• 期刊: NeuroImage
• 影响因子: 5.7
• 作者: Kawai Y;Aoki I;Umeda M;Higuchi T;Kershaw J;Higuchi M;Silva AC;Tanaka C
• 通讯作者: Tanaka C

Detection of necrotic neural response in super-acute cerebral ischemia using activity-induced manganese-enhanced (AIM) MRI.

使用活动诱导锰增强 (AIM) MRI 检测超急性脑缺血中的坏死神经反应。

• 发表时间: 2009
• 期刊: NMR in Biomedicine 23
• 作者: Inoue Y;Aoki I;Mori Kawai Y;Ebisu T;Tanaka C;et al.(10人、10番目)
• 通讯作者: et al.(10人、10番目)