Modification of the Hepatic Mitochondrial Proteome in Response to Ischemic Preconditioning Following Ischemia-Reperfusion Injury of the Rat Steatotic Liver.

大鼠脂肪肝缺血再灌注损伤后肝线粒体蛋白质组的修饰对缺血预处理的反应。

• 批准号: 16591367
• 负责人: NAKANO Hiroshi
• 金额: $ 2.24万
• 依托单位: St.Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591367/
• 关键词: Steatotic liver; Zucker rat; Ischemic preconditioning; Ischemia reperfusion; 2D-DIGE; Proteomics; Mitochoindria; 脂肪肝Zuckerラット; ミトコンドリア蛋白; JC-1; 70%肝切除術; ubiquinol cytochrome C reductase core protein I; superoxide dismutase 2; 2-cystine peroxi

Background/Aim : Ischemic preconditioning (IPC) has been shown to reduce hepatic ischemia-reperfusion (IR) injury of steatotic liver, but the effect of IPC on mitochondrial proteome has not been fully demonstrated. The present study investigated how IPC modifies the mitochondrial proteome after IR injury of the rat steatotic liver.Methods : Zucker steatotic rats were subjected to 25-min portal triad cross-clamping (IR group). In the IPC group, 10-min temporal clamping followed by 10-min reperfusion was performed before the 25-min clamping. The other rats were sham-operated as a control group. Mitochondrial proteome was analyzed 24 hours after IR using two-dimensional differential in-gel electrophoresis and mass spectrometry.Results : Mitochondrial inner-membrane potential and glutathione were lower and serum transaminase was higher in the IPC group than in the IR group. There was a greater degree of up-regulation of the mitochondrial precursor of aldehyde dehydrogenase 2 and alpha-methylacyl-CoA racemase in the IPC group in comparison to the IR group. In contrast, 60S acid ribosomal protein P0, carbonic anhydrase 3, arginase 1, and superoxide dismutase were significantly more down-regulated in the IPC group than in the IR group.Conclusions : A hepato-protective effect was not shown by IPC ; however, IPC caused significant up-or down-regulation of several mitochondrial proteins 24 hours after IR of steatotic liver.

背景/目的：缺血预适应(IPC)可减轻脂肪变性肝脏缺血再灌注(IR)损伤，但其对线粒体蛋白质组的影响尚未完全阐明。本研究观察IPC对脂肪变性大鼠肝脏缺血再灌注损伤后线粒体蛋白质组的影响。方法：Zucker脂肪变性大鼠采用门静脉三联阻断25min(IR组)。IPC组在阻断25min前，先夹闭10min，再灌流10min。其余大鼠均为假手术对照组。结果：IPC组线粒体内膜电位和谷胱甘肽含量低于IR组，血清转氨酶高于IR组。与IR组相比，IPC组线粒体前体醛脱氢酶2和α-甲酰辅酶A外消旋酶表达上调的程度更大。相反，IPC组60S酸性核糖体蛋白P0、碳酸氢酶3、精氨酸酶1和超氧化物歧化酶的表达较IR组明显下调。结论：IPC对肝脏无明显的保护作用，但IPC可显著上调或下调部分线粒体蛋白的表达。

项目成果

A patient undergoing pancreaticoduodenectomy in whom involved common hepatic artery anomalously arising from superior mesenteric artery was removed and reconstructed

一名接受胰十二指肠切除术的患者，其中异常源自肠系膜上动脉的肝总动脉被切除并重建

• 发表时间: 2005
• 期刊: Hepatogastroenterology 52
• 作者: Nakano H;et al.
• 通讯作者: et al.

Pancreaticoduodenectomy in common hepatic artery anomalously arising from the superior mesenteric artery.

异常起源于肠系膜上动脉的肝总动脉胰十二指肠切除术。

• 发表时间: 2005
• 期刊: Hepatogastroenterology 52(66)
• 作者: 中野 浩;ほか
• 通讯作者: ほか

Protein pattern difference in the colon cancer cell lines examined by two-dimensional differential in-gel electrophoresis and mass spectrometry

• DOI: 10.1007/s00595-006-3301-y
• 发表时间: 2006-12-01
• 期刊: SURGERY TODAY
• 影响因子: 2.5
• 作者: Katayama, Masafumi;Nakano, Hiroshi;Otsubo, Takehito
• 通讯作者: Otsubo, Takehito

Modification of the Hepatic Mitochondrial Proteome in Response to Ischemic Preconditioning Following Ischemia-Reperfusion Injury of the Rat Steatotic Liver.Eur Surg Res (in press)

大鼠脂肪肝缺血再灌注损伤后肝线粒体蛋白质组的修饰对缺血预处理的反应。Eur Surg Res（印刷中）

• 发表时间: 2008
• 期刊: Eur Surg Res 40(3)
• 作者: Oshima R;et. al.
• 通讯作者: et. al.

Modification of the Hepatic Mitochondrial Proteome in Response to Ischemic Preconditioning Following Ischemia-Reperfusion Injury of the Rat Steatotic Liver.

大鼠脂肪肝缺血再灌注损伤后肝线粒体蛋白质组的修饰对缺血预处理的反应。

• 发表时间: 2008
• 期刊: Eur Surg Res Eur Surg Res 40
• 作者: Oshima R;et. al.;Nakano H;Oshima R
• 通讯作者: Oshima R