Modification of the Hepatic Mitochondrial Proteome in Response to Ischemic Preconditioning Following Ischemia-Reperfusion Injury of the Rat Steatotic Liver.

背景/目的：缺血预处理（IPC）已被证实可减轻脂肪变性肝缺血再灌注（IR）损伤，但IPC对线粒体蛋白质组的影响尚未完全证实。本研究探讨了IR损伤大鼠脂肪变性肝后，IPC对线粒体蛋白质组的影响。方法：对Zucker脂肪变性大鼠进行25min门脉三联锁断（IR组）。IPC组在25min夹持前，进行10min颞部夹持，10min再灌注。其他大鼠作为对照组进行假手术。用二维凝胶电泳和质谱分析IR后24小时线粒体蛋白质组。结果：IPC组线粒体膜电位和谷胱甘肽低于IR组，血清转氨酶高于IR组。与IR组相比，IPC组线粒体前体醛脱氢酶2和α -甲基酰基辅酶a消旋酶的上调程度更大。相比之下，IPC组60S酸性核糖体蛋白P0、碳酸酐酶3、精氨酸酶1和超氧化物歧化酶的下调明显高于IR组。结论：IPC未显示出肝保护作用；然而，IPC在脂肪变性肝IR后24小时引起几种线粒体蛋白的显著上调或下调。

Background/Aim : Ischemic preconditioning (IPC) has been shown to reduce hepatic ischemia-reperfusion (IR) injury of steatotic liver, but the effect of IPC on mitochondrial proteome has not been fully demonstrated. The present study investigated how IPC modifies the mitochondrial proteome after IR injury of the rat steatotic liver.Methods : Zucker steatotic rats were subjected to 25-min portal triad cross-clamping (IR group). In the IPC group, 10-min temporal clamping followed by 10-min reperfusion was performed before the 25-min clamping. The other rats were sham-operated as a control group. Mitochondrial proteome was analyzed 24 hours after IR using two-dimensional differential in-gel electrophoresis and mass spectrometry.Results : Mitochondrial inner-membrane potential and glutathione were lower and serum transaminase was higher in the IPC group than in the IR group. There was a greater degree of up-regulation of the mitochondrial precursor of aldehyde dehydrogenase 2 and alpha-methylacyl-CoA racemase in the IPC group in comparison to the IR group. In contrast, 60S acid ribosomal protein P0, carbonic anhydrase 3, arginase 1, and superoxide dismutase were significantly more down-regulated in the IPC group than in the IR group.Conclusions : A hepato-protective effect was not shown by IPC ; however, IPC caused significant up-or down-regulation of several mitochondrial proteins 24 hours after IR of steatotic liver.