EFFECT OF ACETOALDEHYDE ON THE GENE EXPRESSION OF TYPE IV COLLAGENASES AND THOSE INHIBITORS IN CULTURED HUMAN ITO-CELLS

我们研究了乙醛（AcCHO）对从手术切除的人肝中分离的培养的Ito细胞中基质金属蛋白酶-2和-9（MMP-2和-9）以及金属蛋白酶组织抑制剂-1，-2和-3（TIMP-1，-2和-3）基因表达的影响。培养的Ito细胞表达MMP-2、TIMP-1和TIMP-3的mRNA。AcCHO对IV型胶原合成无刺激作用，但对MMP-2、TIMP-1和TIMP-3的mRNA水平有抑制作用。培养的人Ito细胞中未检测到MMP-9和TIMP-2的基因表达。TIMP-1和TIMP-3水平的降低可能是MMP-2下降的结果。这些数据表明，抑制MMP-2的基因表达可能有助于增强酒精性肝纤维化中IV型胶原的沉积。

We studied the effect of acetoaldehyde (AcCHO) on gene expression of matrix metalloproteinase-2 and -9(MMP-2 and -9) and tissue inhibitors of metalloproteinase-1, -2 and -3(TIMP-1, -2 and -3) in cultured Ito-cells isolated from surgically resected human liver. Cultured Ito-cells expressed mRNA of MMP-2, TIMP-1 and TIMP-3 respectively. AcCHO did not show any stimulating effect of type IV collagen synthesis, whereas inhibited the mRNA levels of MMP-2, TIMP-1 and -3. The gene expression of MMP-9 and TIMP-2 could not detect in cultured human Ito-cells. Decreased levels TIMP-1 and -3 may be in consequence of decline of MMP-2. These data suggest that suppressed gene expression of MMP-2 may contribute to enhance the type IV collagen deposition in alcoholic liver fibrosis.