Application of in vivo electroporation to DNA immunisation

质粒DNA的体内递送诱导了对编码蛋白疫苗的免疫反应。在包括人类在内的大型动物中，DNA疫苗接种在成为商业现实之前需要进一步改进，至少部分原因是体内表达水平非常低。在体内电穿孔已被证明是提高蛋白质表达水平和提高DNA疫苗效力的有效途径。我们将利用电穿孔增强的体内表达与编码细胞因子的质粒共同递送相结合，以增强和调节绵羊的DNA疫苗。我们将把我们的发现应用于牛病毒性腹泻病毒（BVDV），既作为人类的动物模型，也作为一种经济上重要的牲畜疾病。

The in vivo delivery of plasmid DNA induces immune responses to the encoded protein vaccine. In large animals including humans, DNA vaccination needs to be further improved before becoming a commercial reality, at least partially due to the very low levels of expression in vivo. In vivo electroporation has proven to be an effective way to enhance the level of protein expression and increase DNA vaccine efficacy. We combine enhanced in vivo expression using electroporation with the co-delivery of plasmids encoding cytokines to enhance and modulate DNA vaccine in sheep. We will apply our findings to bovine viral diarrhoea virus (BVDV), both as an animal model for humans and as an economically important diseases of livestock.