Application of in vivo electroporation to DNA immunisation

体内电穿孔在DNA免疫中的应用

• 批准号: LP0561957
• 负责人: Prof Jean-Pierre Scheerlinck
• 金额: $ 28.39万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2006
• 资助国家: 澳大利亚
• 起止时间: 2006-02-15 至 2008-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0561957
• 关键词: Application; vivo; electroporation; DNA; immunisation

The in vivo delivery of plasmid DNA induces immune responses to the encoded protein vaccine. In large animals including humans, DNA vaccination needs to be further improved before becoming a commercial reality, at least partially due to the very low levels of expression in vivo. In vivo electroporation has proven to be an effective way to enhance the level of protein expression and increase DNA vaccine efficacy. We combine enhanced in vivo expression using electroporation with the co-delivery of plasmids encoding cytokines to enhance and modulate DNA vaccine in sheep. We will apply our findings to bovine viral diarrhoea virus (BVDV), both as an animal model for humans and as an economically important diseases of livestock.

质粒DNA的体内递送诱导对编码的蛋白质疫苗的免疫应答。 在包括人类在内的大型动物中，DNA疫苗接种在成为商业现实之前需要进一步改进，至少部分是由于体内表达水平非常低。体内电穿孔已被证明是提高蛋白表达水平和增加DNA疫苗效力的有效方法。我们将联合收割机结合使用电穿孔的增强的体内表达与编码细胞因子的质粒的共递送来增强和调节绵羊中的DNA疫苗。我们将把我们的发现应用于牛病毒性腹泻病毒（BVDV），既作为人类的动物模型，也作为经济上重要的牲畜疾病。