Development of the Simulated Moving-Bed Reactor Equipped with Membrane-Type Enzyme Reactors

膜式酶反应器模拟移动床反应器的研制

• 批准号: 09450291
• 负责人: HASHIMOTO Kenji
• 金额: $ 9.02万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09450291/
• 关键词: Simulated Moving Bed; Reaction and Separation; Chromatographic Reactor; Lactosucrose; beta-Fructofuranosidase; Immobilized Enzyme; Membrane-type enzyme reactor; Numerical Simulation; 模型酵素反応器; 酵素反応; クロマトグラフィー

The objective of this study is to develop a new process by combining the enzyme reactors and simulated moving-bed adsorber and to achieve the high-yield production of lactosucrose (4^G-beta-D-galactosyl sucrose).By carrying out the batch reaction experiments using the enzyme solution, it was found that the transfructosylation followed the ordered bi bi mechanism and the hydrolyses of sucrose and lactosucrose took place simultaneously. The reaction rates were formulated from the obtained reaction mechanism. The reaction experiments using the enzyme immobilized on resin particles as well as the enzyme trapped in the hollow-fiber ultrafiltration membrane were also carried out. By immobilizing the enzyme on the resin, the lactosucrose selectivity was reduced, In the follow-fiber membrane reactor, the mixing conditions reached complete mixing, which slightly reduced the lactosucrose selectivity.The adsorption isotherms and volumetric mass transfer coefficients of each saccharide were determined in single-column breakthrough experiments.Simultaneous reaction and separation experiments were carried out by using the simulated moving-bed reactor with enzyme solution as well as with immobilized enzyme. The selectivity and yield of lactosucrose were 71% and 51% respectively when the enzyme solution was used at 50。C.The lactosucrose selectivity was 60% when the immobilized enzyme was used at 50。C.The results of the numerical simulation of the simulated moving-bed reactor demonstrated that the reverse reaction of the transfructosylation is successfully suppressed by applying the simulated moving-bed technology to this reaction system. It was also found that the sucrose conversion in each reaction column has to be reduced and the number of the reaction columns has to be increased in order to obtain a high yield of lactosucrose in the simulated moving-bed reactor equipped with the immobilized enzyme columns.

本研究的目的是开发一种结合酶反应器和模拟移动床吸附器的新工艺，实现乳糖蔗糖（4^G-β-D-半乳糖基蔗糖）的高产生产。通过使用酶溶液进行间歇反应实验，发现转果糖基化遵循有序bi bi机制，并且蔗糖和半乳糖基蔗糖的水解反应遵循有序bibi机制。 乳糖蔗糖同时发生。根据获得的反应机理制定反应速率。还进行了使用固定在树脂颗粒上的酶以及捕获在中空纤维超滤膜中的酶的反应实验。通过将酶固定在树脂上，降低了乳果糖的选择性，在后续纤维膜反应器中，混合条件达到完全混合，略微降低了乳果糖的选择性。通过单柱突破实验测定了各糖类的吸附等温线和体积传质系数。同时进行了反应和分离实验。 通过使用带有酶溶液以及固定化酶的模拟移动床反应器。酶液在50℃时，乳果糖的选择性和产率分别为71%和51%。固定化酶在50℃时，乳果糖的选择性为60%。模拟移动床反应器的数值模拟结果表明，将模拟移动床技术应用于该反应体系，成功抑制了转果糖基化的逆反应。还发现，为了在配备有固定化酶柱的模拟移动床反应器中获得高产率的乳蔗糖，必须降低每个反应柱中的蔗糖转化率并且必须增加反应柱的数量。