Development of the Simulated Moving-Bed Reactor Equipped with Membrane-Type Enzyme Reactors

膜式酶反应器模拟移动床反应器的研制

• 批准号: 09450291
• 负责人: HASHIMOTO Kenji
• 金额: $ 9.02万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09450291/
• 关键词: Simulated Moving Bed; Reaction and Separation; Chromatographic Reactor; Lactosucrose; beta-Fructofuranosidase; Immobilized Enzyme; Membrane-type enzyme reactor; Numerical Simulation; 模型酵素反応器; 酵素反応; クロマトグラフィー

The objective of this study is to develop a new process by combining the enzyme reactors and simulated moving-bed adsorber and to achieve the high-yield production of lactosucrose (4^G-beta-D-galactosyl sucrose).By carrying out the batch reaction experiments using the enzyme solution, it was found that the transfructosylation followed the ordered bi bi mechanism and the hydrolyses of sucrose and lactosucrose took place simultaneously. The reaction rates were formulated from the obtained reaction mechanism. The reaction experiments using the enzyme immobilized on resin particles as well as the enzyme trapped in the hollow-fiber ultrafiltration membrane were also carried out. By immobilizing the enzyme on the resin, the lactosucrose selectivity was reduced, In the follow-fiber membrane reactor, the mixing conditions reached complete mixing, which slightly reduced the lactosucrose selectivity.The adsorption isotherms and volumetric mass transfer coefficients of each saccharide were determined in single-column breakthrough experiments.Simultaneous reaction and separation experiments were carried out by using the simulated moving-bed reactor with enzyme solution as well as with immobilized enzyme. The selectivity and yield of lactosucrose were 71% and 51% respectively when the enzyme solution was used at 50。C.The lactosucrose selectivity was 60% when the immobilized enzyme was used at 50。C.The results of the numerical simulation of the simulated moving-bed reactor demonstrated that the reverse reaction of the transfructosylation is successfully suppressed by applying the simulated moving-bed technology to this reaction system. It was also found that the sucrose conversion in each reaction column has to be reduced and the number of the reaction columns has to be increased in order to obtain a high yield of lactosucrose in the simulated moving-bed reactor equipped with the immobilized enzyme columns.

为了探索酶反应器与模拟移动床吸附器相结合的生产低聚乳果糖（4^G-beta-D-galactosyl sucrose）的新工艺，采用酶溶液进行分批反应实验，发现果糖基转移反应遵循有序双双机理，蔗糖和低聚乳果糖同时水解。反应速率由所得到的反应机理公式化。同时，利用树脂颗粒固定化酶和中空纤维超滤膜截留酶进行了反应实验。通过将酶固定在树脂上，降低了乳蔗糖的选择性，在后续纤维膜反应器中，混合条件达到完全混合，通过单柱穿透实验测定了各糖的吸附等温线和体积传质系数，并利用模拟移动床进行了反应和分离的同步实验，结果表明，各糖的吸附等温线和体积传质系数均随移动床的移动而增大，但移动床的移动速度和移动床的移动速度不同，移动床的移动速度和移动床的移动速度不同。具有酶溶液以及具有固定化酶的床反应器。当酶液温度为50 ℃时，低聚乳蔗糖的选择性和产率分别为71%和51%。在50 ℃时，低聚乳蔗糖的选择性为60%。对模拟移动床反应器的数值模拟结果表明，将模拟移动床技术应用于该反应体系，成功地抑制了果糖基转移的逆反应。还发现，为了在配备有固定化酶柱的模拟移动床反应器中获得高产率的低聚乳蔗糖，必须降低每个反应柱中的蔗糖转化率，并且必须增加反应柱的数量。