THE EFFECT OF LH-RH ANALOGUE OR/AND TAMOXIFEN ON BONE IN RATS

本文从生化和组织形态两方面研究了促黄体生成素受体类似物或/和他莫昔芬对大鼠骨的影响。实验对象为12周龄雌性Wistar大鼠。这些动物被分成五组。具体分为假手术组、卵巢切除组、促黄体生成素类似物(3.6 mg)组、促黄体生成素类似物(3.6 mg)组、三苯氧胺组(0.01 mg/kg)和黄体生成素类似物(3.6 mg)组。皮下注射促黄体生成素释放激素激动剂。大鼠口服三苯氧胺。骨病的评价以股骨灰分含量减少为特征，组织学上以股骨干骺端骨小梁消失为特征。注射促黄体生成素-RH类似物和去卵巢大鼠出现的代谢性骨障碍在组织形态上是骨质疏松。停用促黄体生成素-RH类似物后，大鼠骨量增加。他莫昔芬不能阻止黄体生成素-促性腺激素类似物引起的骨容量丢失。

The effect of LH-RH analogue or/and Tamoxifen on bone in rats were investigated biochemically and histomorphologically. 12-week old female Wistar rats were in the experiment. The animals were divided into five groups. Specifically, there were sham operation group, ovariectomy group, LH-RH analogue (3.6mg) group, LH-RH analogue(3.6mg), given 0.01mg/kg of Tamoxifen group and LH-RH analogue (3.6mg), given 0.1mg/kg of Tamoxifen group. LH-RH agonist was injected hypodermically. Tamoxifen was given orally to the rats. The evaluation of bone disorders was characterrized by reduction in ash content of the femur and histologically by a disappearance of the trabecular bone in femur metaphysis.The metabolic bone disorders developd in injected LH-RH analogue and ovariectomised rats were osteoporosis histomorphologically. The bone mass in rats after stopped LH-RH analogue was increasing. Tamoxifen could not prevent bone volume loss induced LH-RH analogue.