Expression of Th2-specific chemokines in bronchial asthma

支气管哮喘中Th2特异性趋化因子的表达

• 批准号: 12670415
• 负责人: ISHII Akira
• 金额: $ 2.05万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670415/
• 关键词: bronchial asthma; allergy; Th2 cells; chemokine; TARC; CCL17; MDC; CCL22; CCR4; bronchial epithelial cells; ELISA

We investigated the expression of Th2-specific chemolines, i. e., TARC (thymus and activation regulated chemokine) / CCL 17 and MDC (macrophage-derived chemokine) / CCL22 in bronchial asthma. Bronchial epithelium of asthmatics but not normal exhibited intense immunoreactivity with TARC. MDC immunoreactivty was only weak in both asthmatic and normal epithelium, and no difference of staining intensity was observed in both groups. Bronchial epithelial cell line cells liberated ng/ml quantities of TARC but not MDC in response to combined stimulation with IL-4/TNF-α. (Sekiya. T, et al. J. Immunol.65 : 2205-13, 2000)TARC levels in sputum and serum in asthmatics was increased compared to those in normals. On the other hand, no significant difference was observed in MDC level in both group. (Seliya. T, et al. Allergy, in press)

我们研究了Th2特异性趋化因子的表达，即：例如，TARC/CCL 17和MDC/CCL 22在支气管哮喘中的作用哮喘患者的支气管上皮细胞TARC呈强阳性反应，而正常人的支气管上皮细胞则不呈阳性反应。MDC在哮喘组和正常组上皮中均呈弱阳性表达，两组间染色强度无差异。支气管上皮细胞系细胞在IL-4/TNF-α联合刺激下释放ng/ml量的TARC，但不释放MDC。（关谷。T等，J.Immunol.65：2205 - 13，2000）与正常人相比，哮喘患者痰和血清中的TARC水平增加。另一方面，两组MDC水平无显著差异。（Seliya。T等人，《过敏》，出版中）

项目成果

Sekiya, T., H.Yamada, A.Ishii, K.Hirai., et al.: "Increased levels of a Th2-type CC Chemokines thymus and activation-regulated chemokine (TARC) in induced sputum and serum of asthmatics"Allergy. 57(in press). (2002)

Sekiya, T.、H.Yamada、A.Ishii、K.Hirai. 等人：“哮喘患者诱导痰和血清中 Th2 型 CC 趋化因子胸腺和活化调节趋化因子 (TARC) 水平升高”过敏

Sekiya, T., H.Yamada, A.Ishii, K.Hirai., et al.: "Increased levels of a Th2-type CC Chemokines thymus and activation-regulated chemokine (TARC) in duced sputum and serum of asthmatics"Allergy. 57(in press). (2002)

Sekiya, T.、H.Yamada、A.Ishii、K.Hirai. 等人：“哮喘患者诱导痰和血清中 Th2 型 CC 趋化因子胸腺和活化调节趋化因子 (TARC) 水平升高”过敏

Sekiya, T., M.Miyamasu, A.Ishii, K.Hirai., et al.: "Inducible expression of a Th2-type CC Chemokine thymus and activation regulated chemokine (TARC) by human bronchial epithelial Cells"J. Immunol.. 165. 2205-2213 (2000)

Sekiya, T., M.Miyamasu, A.Ishii, K.Hirai., et al.：“人支气管上皮细胞诱导表达 Th2 型 CC 趋化因子胸腺和活化调节趋化因子 (TARC)”J.

Sakiya, T., M. Miyamasu, A.Ishii, K.Hirai., et al.: "Inducible expression of a Th2-type CC chemokine thymus- and activation-regulated chemokine (TARC) by human bronchial epithelial cells"J. Immunol.. 165. 2205-2213 (2000)

Sakiya, T., M. Miyamasu, A.Ishii, K.Hirai., et al.：“人支气管上皮细胞诱导表达 Th2 型 CC 趋化因子胸腺和激活调节趋化因子 (TARC)”J.

Ohta, K., N.Yamashita, A.ishii, T.Miyamoto., et al.: "In vivo effects of apoptosis in asthma examined by a murine model"Int. Archi. Allergy Immunol.. 124(1-3). 259-261 (2001)

Ohta，K.，N.Yamashita，A.ishii，T.Miyamoto.，等人：“通过小鼠模型检查哮喘中细胞凋亡的体内影响”Int。