Application of Fourier transformation-linear ion trap mass spectrometers to forensic medicine

傅里叶变换线性离子阱质谱仪在法医学中的应用

• 批准号: 23390183
• 负责人: ISHII Akira
• 金额: $ 10.32万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390183/
• 关键词: 法中毒学; 質量分析; オービトラップ; 飛行時間型質量分析計; 自然毒; アマニチン; 合成カンナビノイド; ミフェプリストン; タンデム質量分析; 飛行時間型; 代謝物; UPLC-MS-MS; Orbitrap; Q-TOF; 法医学; 中毒学; 分析化学

The purpose of the project is to develop application methods for identifying and quantitating various drugs and toxins using a Fourier transformation-linear ion trap mass spectrometer, shared in the Faculty. However, under the available LC conditions optimized to proteomic analysis, sufficient separation and reproducibility were not obtained. We thus purchased a quadrupole-time of flight mass spectrometer (Q-TOFMS); further experiments were rendered using this (liquid chromatography) LC-Q-TOFMS system.Using the LC-Q-TOFMS system, amanitins in urine samples were sufficiently separated; the calibration curves showed good linearity in the range of 1 - 5,000 ng/ml. This system has enabled sensitive detection and identification of various synthetic cannabinoids and mifepristone, and their metabolites with high sensitivity. We are now constructing a screening system of 53 natural toxins. Hence, LC-Q-TOFMS will be a useful tool in analyzing various compounds in forensic toxicology.

该项目的目的是开发使用傅里叶变换-线性离子阱质谱仪识别和定量各种药物和毒素的应用方法，并与学院共享。然而，在现有的优化蛋白质组学分析的LC条件下，没有获得足够的分离和重现性。因此，我们购买了四极杆飞行时间质谱仪（Q-TOFMS）；采用该液相色谱LC-Q-TOFMS系统进行进一步实验。采用LC-Q-TOFMS系统，充分分离尿样品中的amamitins；在1 ~ 5000 ng/ml范围内线性良好。该系统对多种合成大麻素和米非司酮及其代谢产物进行了高灵敏度的检测和鉴定。我们现在正在构建一个53种天然毒素的筛选系统。因此，LC-Q-TOFMS将成为法医毒理学中各种化合物分析的有用工具。

项目成果

INCIDENCE OF YOUNG ONSET INSULIN-REQUIRING DIABETES MELLITUS AMONG 18- TO 30-YEAR-OLDS IN DHAKA, BANGLADESH (1994−2003)

孟加拉国达卡 18 至 30 岁青年中发病的胰岛素需求型糖尿病发病率（1994−2003 年）

• DOI: 10.18999/nagjms.74.1-2.149
• 发表时间: 2012
• 期刊: Nagoya Journal of Medical Science
• 影响因子: 0.9
• 作者: Saika Nizam;M. Khalequzzaman;H. Yatsuya;Khanam Pa;Sayeed Ma;Hisao Naito;T. Nakajima
• 通讯作者: T. Nakajima

Metabolism of the newly encountered drug α-pyrrolidino- valerophenone in humans : identification and quantitation of urinary metabolites

新遇到的药物α-吡咯烷-戊苯酮在人体中的代谢：尿代谢物的鉴定和定量

• 发表时间: 2014
• 期刊: Forensic Toxicol
• 影响因子: 2.2
• 作者: Shima N;Katagi M;Kamata H;Matsuta S;Sasaki K;Kamata T;Nishioka H;Miki A;Tastuno M;Zaitsu K;Ishii A;Sato T;Tsuchihashi H;Suzuki K
• 通讯作者: Suzuki K

High-throughput and simultaneous analysis of eight central-acting muscle relaxants in human plasma by ultra-performance liquid chroma-tography-mass spectrometry in the positive and negative modes

采用超高效液相色谱-质谱法在正负模式下高通量同时分析人血浆中的八种中枢作用肌松剂

• 发表时间: 2011
• 期刊: Anal.Bioanal.Chem.
• 作者: Ogawa T;et al
• 通讯作者: et al

Isomeric analysis of synthetic cannabinoids detected as designer drugs

检测为设计药物的合成大麻素的异构体分析

• 发表时间: 2011
• 期刊: Yakugaku Zasshi
• 影响因子: 0.3
• 作者: Uchiyama N;et al
• 通讯作者: et al

Simple and rapid screening for methamphetamine, 3,4-methylenedioxy-methamphetamine (MDMA) and their metabolites in urine using DART (Direct Analysis in Real Time)-TOFMS

使用 DART（实时直接分析）-TOFMS 简单快速地筛查尿液中的甲基苯丙胺、3,4-亚甲二氧基甲基苯丙胺 (MDMA) 及其代谢物

• 发表时间: 2011
• 期刊: Yakugaku Zasshi
• 影响因子: 0.3
• 作者: Kawamura M;et al
• 通讯作者: et al