Elucidation of bio-molecule recognition using semiconductor surface and its application to bioelectronics

阐明利用半导体表面的生物分子识别及其在生物电子学中的应用

• 批准号: 14205007
• 负责人: NIWANO Michio
• 金额: $ 34.94万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14205007/
• 关键词: DNA; hybridization; infrared spectroscopy; bioelectronics; cell; apotosis; 半導体表面; 分子認識; DNAチップ; 脂質分子; 生体機能

In this study, we aimed at giving a function of molecule recognition to a semiconductor surface by forming structurally controlled organic thin film on it and by detecting adsorption state of biomolecules and supramolecules which adsorb onto it using infrared spectroscopy.We need to use heavy water as substitute for ordinary water because DNA, protein, and cells have IR absorption peaks at the same region as the scissors mode of ordinary water. We cannot use general database because of deuterium substituion reaction at part of fuction groups when heavy water is used. Thus, we made new one by comparing results of experimental IR spectra and an ab-initio calculation. We demonstrated (1) highly sensitive detection of DNA hybridization and (2) elucidation of hybridization of DNA immobilized on silicon surface. We found that hybridization reaction strongly influences the C=O stretching vibration mode of bases by comparing IR spectral changes with the ab-initio calculation. We established electrophoresis in a solution and confirmed that electrical potential effectively draws DNA molecules up to silicon surface to enhance its sensitivity. An IR spectrum of drawn DNA molecules differed from one of floating DNA. This spectral change is attributed to protonization of bases. For the first time, we found that electrochemically reaction near the electrode can induce this phenomenon which is a well-known reaction depending on pH of a DNA solution. We demonstrated (5) highly sensitive detection of DNA hybridization using a porous thin film such as porous alumina and porous silicon. Furthermore, we started developing a method of analyzing functions of cells and we observed "apotosis" which is well-known as a designed death using IR spectroscopy and we could trace chemical changes in a cell until it resulted in the cell death. We now promote this study.

在这项研究中，我们的目的是通过在半导体表面形成结构可控的有机薄膜，并通过红外光谱检测吸附在其上的生物分子和超分子的吸附状态，来赋予半导体表面分子识别功能。由于DNA、蛋白质和细胞在与普通水的剪刀模式相同的区域有红外吸收峰，因此需要使用重水来代替普通水。当使用重水时，由于部分官能团上会发生重氢取代反应，所以不能使用通用数据库。因此，我们通过比较实验红外光谱和从头计算的结果，得到了新的结果。我们展示了(1)DNA杂交的高灵敏度检测和(2)固定在硅表面的DNA杂交的阐明。通过比较红外光谱的变化和从头计算，发现杂化反应对碱基的C=O伸缩振动模式有很大的影响。我们建立了溶液中的电泳法，证实了电势能有效地将DNA分子吸引到硅表面，以提高其灵敏度。提取的DNA分子的红外光谱与漂浮DNA的红外光谱不同。这种光谱变化归因于碱基的质子化。我们第一次发现，电极附近的电化学反应可以引起这种现象，这是一种众所周知的反应，取决于DNA溶液的pH值。我们展示了(5)利用多孔性薄膜如多孔性氧化铝和多孔硅进行DNA杂交的高灵敏度检测。此外，我们开始开发一种分析细胞功能的方法，我们使用红外光谱观察到了众所周知的设计死亡的“细胞凋亡”，我们可以追踪细胞中的化学变化，直到它导致细胞死亡。我们现在推动这项研究。

项目成果

DETECTION OF DNA HYBRIDIZATION ON POROUS BY INFRARED SPECTROSCOPY : APPLICATION TO BIOSENSING

通过红外光谱检测多孔 DNA 杂交：在生物传感中的应用

• 期刊: Proceedings of 2004 Joint International Meeting of The Electrochemical Society (in press)
• 作者: Ryo-taro Yamaguchi

Adsorption of naphthalene on a Si(100)-2x1 surface investigated by infrared spectroscopy

红外光谱研究萘在 Si(100)-2x1 表面的吸附

• 发表时间: 2005
• 期刊: Surface Science 576
• 作者: N.Abiko;S.Ogawa;Naofumi Abiko;Satoshi Ogawa;N.Abiko;S.Ogawa;T.Nishikawa;S.Ogawa;Koshi Okamura;石井 久夫;H.Ishii;S.Ogawa;Y.Hosoi;T.Nishikawa;K.Okamura
• 通讯作者: K.Okamura

Masanori Shinohara et al.: "Interaction of hydrogen-terminated Si(100), (110), and (111) surfaces with hydrogen plasma investigated by in situ real-time infrared absorption spectroscopy"Journal of Vacuum Science & Technology A. 21・1. 25-31 (2003)

Masanori Shinohara等人：“通过原位实时红外吸收光谱研究氢封端的Si(100)、(110)和(111)表面与氢等离子体的相互作用”真空科学与技术杂志A.21。 1. 25-31 (2003)

Masanori Shinohara: "Formation and decomposition of Si hydrides during adsorption of Si_2H_6 onto Si(100)(2×1)"Physical Review B. 65. 075319-1-075319-7 (2002)

Masanori Shinohara：“Si_2H_6 吸附到 Si(100)(2×1) 上时 Si 氢化物的形成和分解”Physical Review B. 65. 075319-1-075319-7 (2002)

Masanori Shinohara: "Interaction of hydrogen-terminated Si(100), (110), and (111) surfaces with hydrogen plasma investigated by in situ real-time infrared absorption spectroscopy"Journal of Vacuum Science and Technology. A21. 25-31 (2003)

Masanori Shinohara：“通过原位实时红外吸收光谱研究氢封端的 Si(100)、(110) 和 (111) 表面与氢等离子体的相互作用”真空科学与技术杂志。