Creation of high-quality COaAp-collagen composites for reconstruction and regeneration of alveolar bone

近年来，生物支架材料成为组织工程领域的研究热点。将含镁的功能梯度CO_3磷灰石与胶原混合制成FGMgCO_3磷灰石-胶原复合材料。梯度Mg^<2+>离子对磷灰石晶体的修饰促进了细胞粘附。此外，FGMgCO_3 Ap-胶原复合材料作为支架材料，对骨形成有促进作用。此外，为了开发一种新型的可生物降解的骨架增强生物支架材料，将合成的CO_3Ap与中和的胶原凝胶混合，并将CO_3Ap-胶原混合物冷冻干燥制成具有多孔HAp骨架环的海绵。随着培养时间的延长，纯胶原海绵表面基质物质逐渐增多，几乎完全覆盖海绵表面。苏木精-伊红染色切片证实成骨细胞已充分侵入CO 3Ap-胶原海绵。当这些海绵框架复合物被植入到大鼠的骨膜起重机下时，观察到从颅骨骨膜表面朝向复合物的内核的新产生的骨。这些增强型CO 3Ap-胶原海绵有望作为硬组织支架材料应用于临床治疗。

Recently, scaffold biomaterials have been focused in the tissue engineering field. FGMgCO_3Ap-collagen composite was made by mixing the functionally graded CO_3apatite containing Mg with collagen. Modification of gradational Mg^<2+> ions on the apatite crystals promoted cell adhesion. In addition, as a scaffold material, the FGMgCO_3Ap-collagen composite suggested contributing to bone formation. Mg^<2+> ions may contribute to the bone metabolism of osteoclast and osteoblast action with the integrins at their cell surfaces.Furthermore, to develop a new biodegradable scaffold biomaterial reinforced with a frame, synthesized CO_3Ap was mixed with a neutralized collagen gel and the CO_3Ap-collagen mixtures were lyophilized into sponges in a HAp frame ring with pores. Matrix substances on the pure collagen sponge samples increased with the culture period and the surface of the sponge was almost covered. A sectional view of hematoxylin-eosin staining confirmed that osteoblast cells had well invaded the CO_3Ap-collagen sponge. When these sponge-frame complexes were implanted beneath the periosteum crane of rats, newly created bone was observed toward the inner core of the complex from the surface of the periosteum cranii. These reinforced CO_3Ap-collagen sponges are expected to be used as hard tissue scaffold biomaterials for therapeutic purposes.