Affinity chemical modification and functional control onto carbonate-apatite composite scaffolds

为了开发新型生物可降解支架材料，合成了类骨碳酸磷灰石（CO_3Ap），并制备了CO_3Ap-胶原支架。将rh-BMP-2海绵支架复合物植入大鼠颅骨骨膜下，4周后在颅骨骨膜表面形成了足够的新骨。将含有SVVYGLR肽段的CO_3Ap-胶原海绵移植于大鼠胫骨缺损处，1周后可见大量血管内皮细胞迁移，并可见明显的新生血管形成。因此，对低再生区进行细胞因子、血管生成因子等高功能的修饰，作为组织工程生物材料是有效的。

To develop a new biodegradable scaffold biomaterial, bone-like carbonate apatites (CO_3Ap) were synthesized and CO_3Ap-collagen scaffolds were created. When these sponge-frame complexes with rh-BMP2 were implanted beneath the periosteum cranii of rats, sufficient new bone was created at the surface of the periosteum cranii after 4 wks' implantation. Furthermore, when the CO_3Ap-collagen sponge containing SVVYGLR peptide was implanted as a graft into a tissue defect created in rat tibia, the migration of numerous vascular endothelial cells as well as prominent angiogenesis inside the graft could be detected after 1 week. Thus, the modification of higher function such as cytokine and angiogenesis factors is effective for low regeneration region as tissue engineering biomaterials.