Development of a plasticizer free denture soft lining material

为了研究组织调理剂中邻苯二甲酸酯和乙醇的替代化学品的可行性，对候选乙烯基酯作为增塑剂的雌激素活性、细胞毒性和粘弹性进行了检查。分别用E-screen法和MTS法测定了乙烯基酯的雌激素活性和对人成纤维细胞和角质形成细胞的细胞毒性。将4种原型材料和市售组织调理剂分别作用于胶原培养和组织模型实验中的人成纤维细胞，采用MTS、实时荧光定量PCR和ELISA检测细胞毒性和细胞因子表达。通过初始流动试验和橡胶硬度试验考察了原型材料的粘弹性能。测试的乙烯基酯均未表现出任何雌激素活性。与其他乙烯基酯相比，辛酸乙烯酯（VO）和己二酸二乙烯酯的IC 50值（抑制50%活力所需的化学浓度）较高。由VO制成的原型显示出低水平或与常规材料相似的细胞毒性和细胞因子表达。VO的初始流动类似于市售组织调理剂Shofu组织调理剂的初始流动。VO的橡胶硬度直到30天才随时间推移而变化。由VO制成的原型材料具有生物相容性和良好的粘弹性，因此可能是无邻苯二甲酸酯和乙醇的组织调理剂的可能候选者。

To study the feasibility of alternative chemicals for phthalate and ethyl alcohol in tissue conditioners, candidate vinyl esters as plasticizers were examined for estrogenic activity, cytotoxicity and the viscoelastic properties. The estrogenic activities and cytotoxicity in human fibroblasts and keratinocytes of the vinyl esters were measured in an E-screen assay and MTS assay, respectively. Human fibroblasts in collagen culture and tissue model experiment were exposed to the four prototype materials and commercially available tissue conditioners, and cytotoxicity and the expression of cytokine were determined using MTS, real-time PCR and ELISA assay. The viscoelastic properties of the prototype materials were examined by initial flow test and rubber hardness test. None of the vinyl esters tested exhibited any estrogenic activity. The IC50 values (the chemical concentration required to inhibit viability by 50%) of vinyl octanoate (VO) and divinyl adipate were high compared to the other vinyl esters. The prototype made from VO displayed a low or a similar level of cytotoxicity and expression of cytokine as the conventional materials. The initial flow of VO was similar to that of a commercially available tissue conditioner, shofu tissue conditioner. The rubber hardness of VO did not change as days pass until 30 days. The prototype material made from VO was biocompatible and favorably viscoelastic and therefore might be a possible candidate of phthalate and ethyl alcohol free tissue conditioner.