The structure and dynamics of chromatin harboring specific histone modifications
The structure and dynamics of chromatin harboring specific histone modifications
批准号:
19370076
负责人:
KIMURA Hiroshi
金额:
$11.65万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2007
资助国家:
日本
项目状态:
已结题
起止时间:
2007 至 2009
中文摘要
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英文摘要
To understand the structure and dynamics of specialized chromatin harboring distinct histone modifications, we employed chromatin immunoprecipitation followed by mass spectrometry analysis and live cell imaging by using modification-specific monoclonal antibodies. Using affinity beads coated with different antibodies, distinct sets of proteins were identified as components of chromatin enriched in specific modifications. A method for visualizing specific histone modifications in living cells was also developed.
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DOI:
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发表时间:
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