Optical and Nuclear Imaging of Tumor-related genes

肿瘤相关基因的光学和核成像

• 批准号: 19591434
• 负责人: NAKAMURA Kayoko
• 金额: $ 2.91万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591434/
• 关键词: 放射線; 遺伝子; トランスレーショナルリサーチ; イメージング; 光生命科学

We have developed the simple preparation of Tc-99m-labeled or fluorophore-labeled nanoparticle which was reactive to mRNA in the tumor specifically. Nanoparticle consisted of Antisense-oligonucleotides and streptavidin or biotin. We have also developed second-generation nanoparticles which consisted of Antisense-oligonucleotides and streptavidin or biotin and monoclonal antibody which recognized the tumor specific receptor. HER2, which over expressed in the breast tumor implanted in the thigh of mouse, was viewed very clearly when Tc-99m-labeled nanoparticle was injected intravenously.

我们开发了一种简单的tc -99m标记或荧光团标记的纳米颗粒的制备方法，该纳米颗粒对肿瘤中的mRNA具有特异性反应。纳米粒子由反义寡核苷酸和链亲和素或生物素组成。我们还开发了由反义寡核苷酸和链亲和素或生物素组成的第二代纳米颗粒以及识别肿瘤特异性受体的单克隆抗体。通过静脉注射tc -99m标记的纳米颗粒，可以清楚地观察到HER2在植入小鼠大腿的乳腺肿瘤中过表达。

项目成果

Virus mediated delivery of antisense DNA in cell culture.

病毒介导细胞培养物中反义 DNA 的传递。

• 发表时间: 2008
• 作者: K. Nakamura;H. Muraoka;D. Hnatowich;A. Kubo
• 通讯作者: A. Kubo

Virus mediated delivery of antisense DNA in cell culture

病毒介导的细胞培养中反义 DNA 的传递

• 发表时间: 2007
• 作者: Nakamura K;Muraoka H;Hnatowich D;Kubo A
• 通讯作者: Kubo A

ANTISENSE AND PRETARGETING OPTICAL IMAGING

反义和预瞄准光学成像

• 发表时间: 2008

In vivo delivery of antisense MORF oligomer by MORF/carrier streptavidin nanoparticles.

• DOI: 10.1089/cbr.2009.0624
• 发表时间: 2009-10
• 期刊: Cancer biotherapy & radiopharmaceuticals
• 影响因子: 3.4
• 作者: Yi Wang;Xinrong Liu;Kayoko Nakamura;Ling Chen;M. Rusckowski;D. Hnatowich

Auger Radiation-Induced, Antisense-Mediated Cytotoxicity of Tumor Cells Using a 3-Component Streptavidin-Delivery Nanoparticle with In^<111>.

使用具有 In^<111> 的 3 组分链霉亲和素递送纳米颗粒进行俄歇辐射诱导的反义介导的肿瘤细胞细胞毒性。

• 发表时间: 2009
• 期刊: Journal of Nuclear Medicine 50
• 作者: Liu X;Wang Y;Nakamura K;Hnatowich DJ.;et al
• 通讯作者: et al