Elucidation of bacterial pathogenesis system based on a silkworm infection model

基于家蚕感染模型阐明细菌致病系统

• 批准号: 20390021
• 负责人: SEKIMIZU Kazuhisa
• 金额: $ 12.4万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390021/
• 关键词: カイコ; 病原性; 病原性因子; コロニースプレッデング; 病院分離型MRSA; 市中感染型MRSA; psm-mec遺伝子; 可動遺伝要素; SCCmec; バイオフィルム; 黄色ブドウ球菌; 自然免疫; 脂質輸送

In this study, we performed to solve molecular function of CvfB (conserved virulence factor B), which was identified as a virulence regulatory factor using silkworm infection model. CvfB has three S1 RNA binding domain and a Winged-Helix domain revealed by crystal structure at 1.4Å resolution. Moreover, we uncovered the RNA binding activity of CvfB is required for hemolysin production in Staphylococcus aureus.

本研究利用家蚕感染模型，对被鉴定为毒力调控因子的CvfB(保守毒力因子B)的分子功能进行了研究。CvfB具有3个S1RNA结合域和1个Winged-Helix结构域，其晶体结构显示其1.4kD的Å分辨率。此外，我们还发现CvfB的RNA结合活性是金黄色葡萄球菌产生溶血素所必需的。

项目成果

新規環状ペプチド化合物とその製造方法及び感染症治療薬

新型环肽化合物、其生产方法以及感染性疾病治疗

• 发表时间: 2010

Novel RNA polymerase II mutation suppresses transcriptional fidelity and oxidative stress sensitivity in rpb9Delta yeast

新型 RNA 聚合酶 II 突变抑制 rpb9Delta 酵母的转录保真度和氧化应激敏感性

• 发表时间: 2010
• 期刊: Genes Cells 15
• 作者: Koyama H;Ueda T;Ito T;Sekimizu K
• 通讯作者: Sekimizu K

Evaluation of Target Specificity of Antibacterial Agents Using Staphylococcus aureus ddlA Mutants and d-Cycloserine in a Silkworm Infection Model

• DOI: 10.1128/aac.00200-09
• 发表时间: 2009-09
• 期刊: Antimicrobial Agents and Chemotherapy
• 影响因子: 4.9
• 作者: K. Kurokawa;H. Hamamoto;M. Matsuo;S. Nishida;N. Yamane;B. Lee;K. Murakami;H. Maki;K. Sekimizu

Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.

• DOI: 10.1371/journal.ppat.1001267
• 发表时间: 2011-02-03
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Kaito C;Saito Y;Nagano G;Ikuo M;Omae Y;Hanada Y;Han X;Kuwahara-Arai K;Hishinuma T;Baba T;Ito T;Hiramatsu K;Sekimizu K
• 通讯作者: Sekimizu K

A Novel Gene, fudoh, in the SCCmec Region Suppresses the Colony Spreading Ability and Virulence of Staphylococcus aureus

• DOI: 10.1371/journal.pone.0003921
• 发表时间: 2008-12-11
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Kaito, Chikara;Omae, Yosuke;Sekimizu, Kazuhisa
• 通讯作者: Sekimizu, Kazuhisa