Diagnosis of Oral Intraepithelial Neoplasia and Study for Mucosal Tissue Stem Cells Using Our Original Tissue Q-FISH Method.

我们可以使用我们独创的组织Q-FISH方法测量载玻片上每组细胞的端粒长度。在对口腔粘膜的分析中，我们可以确认基底层细胞是上皮层中端粒最长的，这表明组织干细胞位于基底层。我们还可以证明一些异常增生性病变属于原位癌，因为端粒较短，与粘膜癌的模式相同。癌旁或白斑旁的上皮细胞端粒明显短小，染色体不稳定，可能是真正的癌前病变。

We can measure the teIomere length in each cell group on the slide by using our original tissue Q-FISH method. In analysis of oral mucosa, we could confirm that the basal cells have the longest telomeres among the epithelial layer, and that suggests the tissue stem cells are reside in the basal layer. We could also have proved some dysplastic lesions as kinds of carcinoma in situ, because of having short telomeres as same pattern as mucosal carcinomas. The epithelia adjacent to carcinomas or some leukoplakias have significantly short telomeres and chromosomal instabilities, therefore, these lesions might be the true precancerous lesions.