Investigation of a cytosolic sulfur transfer pathway for tRNA thio-modification and biosynthesis of sulfur-containing molecules

用于 tRNA 硫代修饰和含硫分子生物合成的胞质硫转移途径的研究

• 批准号: 21570150
• 负责人: NAKAI Yumi
• 金额: $ 3.08万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570150/
• 关键词: tRNA硫黄修飾; 酵母; ミトコンドリア; サイトゾル; 鉄硫黄クラスター; システインデスルフラーゼ; tRNA修飾; ロダネーゼ; ユビキチン様タンパク質; 硫黄付加; 含硫小分子; チオカルボキシル基; 硫黄運搬

We found that ubiquitin-like protein Urm1 and ubiquitin-activating enzyme-like protein Uba4 were strictly required for the s2 modification. The carboxyl-terminal glycine residue of Urm1 was critical for the s2 modification, indicating direct involvement of the unique ubiquitin-related system in this process. We also demonstrated that the s2 and mcm5 modifications in cytosolic tRNAs influence each other's efficiency. Taken together, our data indicate that the s2 modification of cytosolic tRNAs is a more complex process that requires additional unidentified components.

我们发现，泛素样蛋白Urm1和泛素激活酶样蛋白Uba4是严格需要的s2修饰。Urm1的羧基末端甘氨酸残基对于S2修饰是至关重要的，这表明独特的泛素相关系统直接参与了这一过程。我们还证明了胞质tRNA中的s2和mcm 5修饰会影响彼此的效率。总之，我们的数据表明，胞质tRNA的S2修饰是一个更复杂的过程，需要额外的未鉴定的成分。

项目成果

Arabidopsis cytosolic Nbp35 homodimer can assemble both [2Fe-2S] and [4Fe-4S] clusters in two distinct domains

• DOI: 10.1016/j.bbrc.2008.11.138
• 发表时间: 2009-01-23
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kohbushi, Hirokazu;Nakai, Yumi;Nakai, Masato
• 通讯作者: Nakai, Masato

酵母の鉄硫黄クラスター生合成とtRNA硫黄修飾の関連について

关于酵母铁硫簇生物合成与tRNA硫修饰的关系

• 发表时间: 2009
• 作者: 西毅;久野悠;山口明人;Sohma H;中井由実.中井正人.林秀行
• 通讯作者: 中井由実.中井正人.林秀行

Proteins required for thio-modification of tRNAs participate in the biosyntheses of sulfur-containing cofactors

tRNA 硫代修饰所需的蛋白质参与含硫辅因子的生物合成

• 发表时间: 2011
• 作者: 土谷佳弘;浅野知一郎;中山啓子;加藤友久;Michael Karin;鎌田英明;Yumi Nakai・Akiko Harada・Yasuyuki Hashiguchi・Takeshi Murakawa・Masato Nakai・Hideyuki Hayashi
• 通讯作者: Yumi Nakai・Akiko Harada・Yasuyuki Hashiguchi・Takeshi Murakawa・Masato Nakai・Hideyuki Hayashi