Investigation of role of glial cells in glaucomatous optic neuropathy and searching glial cell-related treatment for glaucoma

胶质细胞在青光眼性视神经病变中的作用研究及寻找胶质细胞相关的青光眼治疗方法

• 批准号: 21592220
• 负责人: KASHIWAGI Kenji
• 金额: $ 2.91万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592220/
• 关键词: 緑内障; グルタミン酸; グルタミン酸トランスポーター; 眼グリア細胞; 網膜神経節細胞; 遺伝子改変マウス; グリア細胞; 神経保護; 神経再生; ミュラー細胞; 視神経アストロサイト; セロトニントランスポーター

I clarified the following points ; Different glutamate transporters(GLT) were differently involved in glial cell-related effect on RGC. Major GLT, GLAST exerted neuroprotective effect on RGC, while GLT-1 exerted effect on promoting neuritis. Muller cells, major retinal glial cells, showed a protective effect on retinal ganglion cells(RGCs), while astrocytes in optic nerve did promoting effect of neurites. Several mRNAs influenced to RGC survival or neurite elongation were identified as key mRNAs for GLT-involved RGC effects. EGFR, Gabbr2, Agtr1a showed protective effect, while Id3、Egr1, Gja1p, App, Nr2f2 mRNAs were involved in RGC death. I developed an animal model that enables to observe axonal flow of lived rat retina.

阐明了以下几点：不同的谷氨酸转运体（GLT）参与了胶质细胞对RGC的影响。主要GLT、GLAST对RGC有神经保护作用，而GLT-1有促进神经炎的作用。视网膜主要的胶质细胞Muller细胞对视网膜神经节细胞（RGCs）有保护作用，而视神经中的星形胶质细胞对神经突起有促进作用。几种影响RGC存活或轴突伸长的mRNA被鉴定为GLT参与RGC效应的关键mRNA。EGFR、Gabbr 2、Agtr 1a对RGC的死亡有保护作用，Id 3、Egr 1、Gja 1 p、App、Nr 2f 2 mRNA参与RGC的死亡。我开发了一种动物模型，能够观察活体大鼠视网膜的轴突流动。

项目成果

Longitudinal Changes in Anterior Chamber Configuration in Eyes With Open-Angle Glaucoma and Associated Factors

• DOI: 10.1097/ijg.0b013e31821206b1
• 发表时间: 2012-06-01
• 期刊: JOURNAL OF GLAUCOMA
• 影响因子: 2
• 作者: Pan, Zhiying;Furuya, Toshie;Kashiwagi, Kenji
• 通讯作者: Kashiwagi, Kenji

眼科遠隔診断システム

眼科远程诊断系统

• 发表时间: 2008

Estrogen receptor beta gene polymorphism and intraocular pressure elevation in female patients with primary open-angle glaucoma

女性原发性开角型青光眼患者雌激素受体β基因多态性与眼压升高的关系

• 发表时间: 2010
• 期刊: Am J Ophthalmol
• 影响因子: 4.2
• 作者: Mabuchi F;Sakurada Y;Kashiwagi K;Yamagata Z;Iijima H;Tsukahara S
• 通讯作者: Tsukahara S

Lack of associaton between p53 gene polymorphisms and primary open angle glaucoma in the Japanese population.

日本人群中 p53 基因多态性与原发性开角型青光眼之间缺乏关联。

• 发表时间: 2009
• 期刊: Molecular Vision VOL.15
• 作者: 岩見史子;岩田英嗣;高橋明子;安間哲宏;大岩和博;川野健一;足立麻世;伊藤逸毅;寺崎浩子;Mabuchi F
• 通讯作者: Mabuchi F

Association of microsatellite polymorphisms of the GPDS1 locus with normal tension glaucoma inthe Japanese population.

GPDS1 基因座的微卫星多态性与日本人群正常眼压性青光眼的关联。

• 发表时间: 2009
• 期刊: Clinical Ophthalmology 3
• 作者: Sasamoto Y;Oshima Y;Miki A;Wakabayashi T;Song D;Matsushita K;Hamasaki T;Nishida K;間渕文彦;Nakamura K
• 通讯作者: Nakamura K