Cryopreservation and improvement of developmental ability of porcine primordial oocytes

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Primordial follicles act as stores for ovarian follicles and are a potential resource of oocytes for medical, agricultural and zoological purposes. In the present study, we have demonstrated that fusion of cytoplasts prepared from competent porcine oocytes enhances the developmental ability of oocytes derived from porcine primordial follicles grafted into nude mice. We also have explored a vitrification protocol that allows porcine ovary tissue to maintain its ability to produce antral follicles after xenografting. Oocytes retrieved from porcine xenografts were proven to have the abilities of in-vitro maturation and fertilization. These methods are necessary for utilization of primordial oocytes in large mammals